Tumor Protein P53 (TP53) (pSer46) antibody

Antigen: Tumor Protein P53 (TP53)

Synonyms: p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53

Binding Site: pSer46

Clonality: Monoclonal (I117-1091)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Catalog no.: ABIN967583

Additional Information

Alternative name: p53

Immunogen: Phosphorylated peptide corresponding to the region including Serine 46 of p53

Format: Liquid

Isotype: IgG1, kappa

Clone: I117-1091

Description: The p53 protein is critical to regulation of normal cell growth and is a suppressor of tumor cell proliferation. Inactivation of p53 by a number of mechanisms, such as missense mutations or interaction with oncogenic viral or cellular proteins, can result in tumor progression. Mutations and/or allelic loss of the p53 gene are associated with a wide variety of human tumors. Known to have a role in transcriptional regulation, p53 suppresses various promoters containing TATA elements in an apparently sequence-independent fashion. p53 also binds to DNA in a sequence-specific manner via recognition of a 20-bp consensus-binding site. This interaction stimulates the expression of genes downstream of the p53 binding site. A number of genes that contain p53-binding sites have been identified, including MDM2, GADD45, and muscle creatine kinase. MDM2 mediates feedback inhibition of p53, which is prevented by phosphorylations of p53 amino-terminal serines and threonines. UV radiation-induced phosphorylation of Serine 46 (S46) is mediated by homeodomain-interacting protein kinase-2 (HIPK2) and is involved in the promotion of apoptosis. Other stress stimuli mediate S46 phosphorylation in p53 via as yet unidentified kinase(s).
The I117-1091 mAb recognizes p53 phosphorylated at S46.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 53 kDa

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

Western blot analysis of p53 (pS46). Lysates from control (First Panel) and UV-irradiated (Second Panel) A431 human carcinoma cells were probed with purified Mouse anti-p53 (pS46) at concentrations of 0.25, 0.125, and 0.06 µg/ml (Lanes 1, 4), (Lanes 2, 5), and (Lanes 3, 6), respectively. p53 (pS46) is identified as a strong band of 53 kDa in the treated cells.

p53 (pS46) staining on human colon carcinoma. The colon carcinoma tissue was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (first panel) or treated with a phosphatase to eliminate all phosphorylation (Second Panel). The tissue sections were stained with purified Mouse anti-p53 (pS46) with Hematoxylin counterstaining. Original magnification: 20X.

Cells treated with a phosphatase to eliminate all phosphorylation

Publications

De Milito, Aleman, Marenzi et al.: "Plasma levels of soluble CD27: a simple marker to monitor immune activation during potent antiretroviral therapy in HIV-1-infected subjects." in: Clinical and experimental immunology, Vol. 127, Issue 3, pp. 486-94, 2002 (PubMed).

Product: Hofmann, Möller, Sirma et al.: "Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2." in: Nature cell biology, Vol. 4, Issue 1, pp. 1-10, 2002 (PubMed).

DOrazi, Cecchinelli, Bruno et al.: "Homeodomain-interacting protein kinase-2 phosphorylates p53 at Ser 46 and mediates apoptosis." in: Nature cell biology, Vol. 4, Issue 1, pp. 11-9, 2002 (PubMed).

Saito, Goodarzi, Higashimoto et al.: "ATM mediates phosphorylation at multiple p53 sites, including Ser(46), in response to ionizing radiation." in: The Journal of biological chemistry, Vol. 277, Issue 15, pp. 12491-4, 2002 (PubMed).

Di Stefano, Blandino, Sacchi et al.: "HIPK2 neutralizes MDM2 inhibition rescuing p53 transcriptional activity and apoptotic function." in: Oncogene, Vol. 23, Issue 30, pp. 5185-92, 2004 (PubMed).

Bode, Dong: "Post-translational modification of p53 in tumorigenesis." in: Nature reviews. Cancer, Vol. 4, Issue 10, pp. 793-805, 2004 (PubMed).