Images for product: anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody
Western blot analysis of ZAP70 (pY493). Lysates from control (left panel) and hydrogen peroxide-treated (right panel) Jurkat cells were probed with purified Mouse anti-ZAP70 (pY493) at concentrations of 8.0 (lanes 1,4), 4.0 (lanes 2,5), and 2.0 myg/ml (Lanes 3,6). ZAP70 (pY493) is identified as a strong band of 70 kDa in the treated cells.
ZAP70 staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column). The tissue sections were stained with either purified Mouse anti-ZAP-70 (ABIN967848 or ABIN967849, top row) or purified Mouse anti-ZAP70 (pY493) (bottom row) with Hematoxylin counterstaining. Original magnification: 20X.