zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody

Antigen: zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyms: SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70

Binding Site: pTyr493

Clonality: Monoclonal (1a)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Catalog no.: ABIN967585

Additional Information

Alternative name: ZAP-70

Format: Liquid

Isotype: IgG1, kappa

Clone: 1a

Description: The 70-kDa zeta chain-associated protein (ZAP70) is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both ZAP70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyrosine 493 (Y493) in the regulatory loop of the kinase domain leading to upregulation of ZAP70 kinase activity. The significance of ZAP70 activation in mediating TCR signal transduction has been confirmed by showing that ZAP70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP70 kinase domain which affect the stability of the protein and TCR signaling. The 1a/Zap70 (pY493) antibody recognizes ZAP70 phosphorylated at Y493.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 70 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Signaling, Tags/Labels, Immunology, Cancer

Restrictions: For Research Use only

Images

Western blot analysis of ZAP70 (pY493). Lysates from control (left panel) and hydrogen peroxide-treated (right panel) Jurkat cells were probed with purified Mouse anti-ZAP70 (pY493) at concentrations of 8.0 (lanes 1,4), 4.0 (lanes 2,5), and 2.0 myg/ml (Lanes 3,6). ZAP70 (pY493) is identified as a strong band of 70 kDa in the treated cells.

ZAP70 staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column). The tissue sections were stained with either purified Mouse anti-ZAP-70 (ABIN967848 or ABIN967849, top row) or purified Mouse anti-ZAP70 (pY493) (bottom row) with Hematoxylin counterstaining. Original magnification: 20X.

Publications

Product: Chan, Dalton, Johnson et al.: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995 (PubMed).

Chan, Kadlecek, Elder et al.: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994 (PubMed).

Mège, Di Bartolo, Germain et al.: "Mutation of tyrosines 492/493 in the kinase domain of ZAP-70 affects multiple T-cell receptor signaling pathways." in: The Journal of biological chemistry, Vol. 271, Issue 51, pp. 32644-52, 1997 (PubMed).