zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody

Details for Product No. ABIN967585
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms zap70, MGC80670, MGC88962, ZAP70, 70kDa, AI327364, Srk, TZK, ZAP-70, mrtle, mur, SRK, STCD, STD
(48), (47), (42), (29), (23), (21), (21), (19), (15), (15), (15), (7), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(488), (180), (125), (3), (2), (1)
(319), (148), (15), (8), (3)
Clonality (Clone)
Monoclonal ()
(34), (29), (22), (14), (10), (9), (5), (5), (4), (4), (4), (4), (4), (4), (2), (1)
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
(303), (166), (152), (98), (64), (46), (40), (28), (13), (12), (6), (5), (4), (3), (2), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States ( )
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Clone 1a
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name ZAP-70 (ZAP70 Antibody Abstract)
Background The 70-kDa zeta chain-associated protein (ZAP70) is a protein tyrosine kinase (PTK) that associates with the zeta subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both ZAP70 and Syk are recruited to the phosphorylated CD3 and zeta subunits after TCR stimulation. TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyrosine 493 (Y493) in the regulatory loop of the kinase domain leading to upregulation of ZAP70 kinase activity. The significance of ZAP70 activation in mediating TCR signal transduction has been confirmed by showing that ZAP70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID). This is due to mutations affecting the ZAP70 kinase domain which affect the stability of the protein and TCR signaling. The 1a/Zap70 (pY493) antibody recognizes ZAP70 phosphorylated at Y493.
Molecular Weight 70 kDa
Research Area Signaling, Tags/Labels, Immunology, Cancer

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody Western blot analysis of ZAP70 (pY493). Lysates from control (left panel) and hydroge...
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody (2) ZAP70 staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate soluti...
Product cited in: Mège, Di Bartolo, Germain et al.: "Mutation of tyrosines 492/493 in the kinase domain of ZAP-70 affects multiple T-cell receptor signaling pathways." in: The Journal of biological chemistry, Vol. 271, Issue 51, pp. 32644-52, 1997 (PubMed).

Chan, Kadlecek, Elder et al.: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994 (PubMed).

Chan, Dalton, Johnson et al.: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995 (PubMed).

Validation Images
Catalog No. ABIN967585
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)
Did you look for something else?