Platelet-Derived Growth Factor Receptor, beta Polypeptide (PDGFRB) (pTyr771) antibody

Antigen: Platelet-Derived Growth Factor Receptor, beta Polypeptide (PDGFRB)

Binding Site: pTyr771

Clonality: Monoclonal (J23-618)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Catalog no.: ABIN967593

Additional Information

Alternative name: CD140b

Immunogen: Phosphorylated Human PDGFRbeta Peptide

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG1, kappa

Clone: J23-618

Description: Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRalpha (CD140a) and/or PDGFRbeta (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to alphaalpha PDGFR, PDGF-AB binds to both alphaalpha and alphabeta PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-gamma.
The J23-618 monoclonal antibody recognizes the phosphorylated tyrosine 771 (pY771) in the kinase insert domain of CD140b. pY771 interacts with GTPase-activating protein, a negative regulator of Ras, and weakly with Shc, which indirectly promotes the activation of Ras.
Synonyms: CD140b (pY771)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 180 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Western blot analysis of PDGFRbeta (pY771). Lysates from control (First Panel) and PDGF-treated (Second Panel) NIH/3T3 mouse embryonic fibroblasts were probed with purified mouse anti-PDGFRbeta (CD140b) (pY771) at concentrations of 0.016 (lanes 1 and 4), 0.008 (lanes 2 and 5), and 0.004 µg/ml (lanes 3 and 6). PDGFRbeta (pY771) is identified as a band of 180 kDa in the treated cells.

PDGFRbeta (pY771) staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with Retrievagen A buffer, the sections were either left untreated (First Panel) or treated with a phosphatase to eliminate all phosphorylation (Second Panel). The tissue sections were stained with purified Mouse anti-PDGFRbeta (CD140b) (pY771) with Hematoxylin counterstaining. Original magnification: 20X.

Cells treated with a phosphatase to eliminate all phosphorylation

Publications

Product: Claesson-Welsh: "Platelet-derived growth factor receptor signals." in: The Journal of biological chemistry, Vol. 269, Issue 51, pp. 32023-6, 1995 (PubMed).

Liu, Oh, Horner et al.: "Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains." in: The Journal of biological chemistry, Vol. 272, Issue 11, pp. 7211-22, 1997 (PubMed).

Ekman, Kallin, Engström et al.: "SHP-2 is involved in heterodimer specific loss of phosphorylation of Tyr771 in the PDGF beta-receptor." in: Oncogene, Vol. 21, Issue 12, pp. 1870-5, 2002 (PubMed).