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Details for Product No. ABIN967593

Platelet-Derived Growth Factor Receptor, beta Polypeptide (PDGFRB) (pTyr771) antibody

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Antigen
Synonyms AI528809, CD140b, PDGFR-1, Pdgfr, CD140B, IBGC4, JTK12, PDGFR, PDGFR1
Epitope
»Alternatives pTyr771
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
Pubmed 3 references available
Catalog no. ABIN967593
Quantity 0.1 mg
Price
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Immunogen Phosphorylated Human PDGFRbeta Peptide
Clone J23-618
Isotype IgG1, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name CD140b
Background Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRalpha (CD140a) and/or PDGFRbeta (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to alphaalpha PDGFR, PDGF-AB binds to both alphaalpha and alphabeta PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-gamma.
The J23-618 monoclonal antibody recognizes the phosphorylated tyrosine 771 (pY771) in the kinase insert domain of CD140b. pY771 interacts with GTPase-activating protein, a negative regulator of Ras, and weakly with Shc, which indirectly promotes the activation of Ras.
Synonyms: CD140b (pY771)
Molecular Weight 180 kDa
Research Area CD Antigens, Surface Receptors of Immune Cells
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Product cited in: Claesson-Welsh: "Platelet-derived growth factor receptor signals." in: The Journal of biological chemistry, Vol. 269, Issue 51, pp. 32023-6, 1995 (PubMed).

Liu, Oh, Horner et al.: "Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains." in: The Journal of biological chemistry, Vol. 272, Issue 11, pp. 7211-22, 1997 (PubMed).

Ekman, Kallin, Engström et al.: "SHP-2 is involved in heterodimer specific loss of phosphorylation of Tyr771 in the PDGF beta-receptor." in: Oncogene, Vol. 21, Issue 12, pp. 1870-5, 2002 (PubMed).

Alternatives for antigen "Platelet-Derived Growth Factor Receptor, beta Polypeptide (PDGFRB)", type "Antibodies"
Hosts (268), (46), (18), (4), (3)
Reactivities (312), (195), (157), (72), (72), (36), (24), (1)
Applications (188), (104), (87), (80), (57), (36), (33), (18), (12), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Conjugates (12), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (1)
Epitopes (24), (23), (21), (16), (16), (14), (14), (12), (12), (7), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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