Western blot analysis of PDGFRbeta (pY771). Lysates from control (First Panel) and PDGF-treated (Second Panel) NIH/3T3 mouse embryonic fibroblasts were probed with purified mouse anti-PDGFRbeta (CD140b) (pY771) at concentrations of 0.016 (lanes 1 and 4), 0.008 (lanes 2 and 5), and 0.004 µg/ml (lanes 3 and 6). PDGFRbeta (pY771) is identified as a band of 180 kDa in the treated cells.
PDGFRbeta (pY771) staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with Retrievagen A buffer, the sections were either left untreated (First Panel) or treated with a phosphatase to eliminate all phosphorylation (Second Panel). The tissue sections were stained with purified Mouse anti-PDGFRbeta (CD140b) (pY771) with Hematoxylin counterstaining. Original magnification: 20X.