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PDGFRB antibody (Platelet-Derived Growth Factor Receptor, beta Polypeptide) (pTyr771)

Details for Product anti-PDGFRB Antibody No. ABIN967593, Supplier: Log in to see
Antigen
  • AI528809
  • CD140B
  • CD140b
  • IBGC4
  • JTK12
  • PDGFR
  • Pdgfr
  • PDGFR-1
  • PDGFR1
Alternatives
anti-Human PDGFRB antibody for Immunofluorescence
Epitope
pTyr771
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Reactivity
Human, Mouse (Murine)
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This PDGFRB antibody is un-conjugated
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Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
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Supplier
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Brand BD Pharmingen™
Immunogen Phosphorylated Human PDGFRbeta Peptide
Clone J23-618
Isotype IgG2a, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD140b (PDGFRB Antibody Abstract)
Background Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRalpha (CD140a) and/or PDGFRbeta (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to alphaalpha PDGFR, PDGF-AB binds to both alphaalpha and alphabeta PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-gamma.
The J23-618 monoclonal antibody recognizes the phosphorylated tyrosine 771 (pY771) in the kinase insert domain of CD140b. pY771 interacts with GTPase-activating protein, a negative regulator of Ras, and weakly with Shc, which indirectly promotes the activation of Ras.
Synonyms: CD140b (pY771)
Molecular Weight 180 kDa
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-PDGFRB antibody (Platelet-Derived Growth Factor Receptor, beta Polypeptide) (pTyr771) (ABIN967593) Western blot analysis of PDGFRbeta (pY771). Lysates from control (First Panel) and PD...
 image for anti-PDGFRB antibody (Platelet-Derived Growth Factor Receptor, beta Polypeptide) (pTyr771) (ABIN967593) PDGFRbeta (pY771) staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervan...
 image for anti-PDGFRB antibody (Platelet-Derived Growth Factor Receptor, beta Polypeptide) (pTyr771) (ABIN967593) Cells treated with a phosphatase to eliminate all phosphorylation
Product cited in: Ekman, Kallin, Engström, Heldin, Rönnstrand: "SHP-2 is involved in heterodimer specific loss of phosphorylation of Tyr771 in the PDGF beta-receptor." in: Oncogene, Vol. 21, Issue 12, pp. 1870-5, 2002 (PubMed).

Liu, Oh, Horner, Rogers, Schnitzer: "Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains." in: The Journal of biological chemistry, Vol. 272, Issue 11, pp. 7211-22, 1997 (PubMed).

Claesson-Welsh: "Platelet-derived growth factor receptor signals." in: The Journal of biological chemistry, Vol. 269, Issue 51, pp. 32023-6, 1995 (PubMed).