Images for product: anti-Caveolin 2 (CAV2) (pTyr27) antibody
Western blot analysis of Caveolin 2 (pY27) in human endothelial cells. Lysates from pervanadate-stimulated endothelial cells (ABIN968630) were either treated with 50 µg/ml alkaline phosphatase (ICN Biomedicals) for 30 minutes at 37°C (left lane) or untreated (right lane), then probed with purified 40/Caveolin 2 mAb at 0.5 µg/ml. Caveolin 2 (pY27) is identified as a band of 20 kDa in the untreated lysate.
Caveolin 2 (pY27) staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (first panel) or treated with a phosphatase to eliminate all phosphorylation (second panel). The tissue sections were stained with purified Mouse anti-Caveolin 2 (pY27) with Hematoxylin counterstaining. Original magnification: 20X.