Caveolin 2 (CAV2) (pTyr27) antibody
|Synonyms||CAV, MGC12294, AI447843, MGC72322, Cav-2, CAV2, CAV-2, zgc:86696, wu:fc52e01, Caveolin-2, cav, MGC108281, caveolin-2, cav2, cav-2|
Alternatives Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Fluorescence Microscopy (FM)
|4 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||Product not available in this region.|
|Alternative name||Caveolin 2|
|Immunogen||Phosphorylated Human Caveolin 2|
The caveolins are a family of transmembrane proteins that are required for the structural integrity and functions of non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. The intracellular functions of caveolae include roles in cholesterol homeostasis, signal transduction, and vesicle transport. Caveolins 1 and 2 form hetero-oligomers in the endoplasmic reticulum, Golgi complex and trans-Golgi-derived transport vesicles in most tissues. The highest levels of these complexes are found in adipocytes, fibroblasts, and endothelial, epithelial, and smooth muscle cells. Upon phosphorylation at tyrosine 19 or 27, caveolin 2 dissociates from caveolin 1, and the two phosphorylated forms of caveolin 2 localize to distinct areas of the cell. There are reports that internalization of caveolae may be regulated by tyrosine phosphorylation of caveolin 2.
The 40/Caveolin 2 monoclonal antibody recognizes the phosphorylated tyrosine 27 (pY27) of caveolin 2.
1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||20 kdA|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||The antibody should be kept at -20°C for long term storage, and it may be kept at 4°C for short term storage.|
|Research Area||Cardiovascular, Atherosclerosis, Organelles|
|Restrictions||For Research Use only|
Conner, Schmid: "Regulated portals of entry into the cell." in: Nature, Vol. 422, Issue 6927, pp. 37-44, 2003 (PubMed).
Williams, Lisanti: "The caveolin proteins." in: Genome biology, Vol. 5, Issue 3, pp. 214, 2004 (PubMed).
Wang, Lee, Capozza et al.: "Tyrosine phosphorylation of caveolin-2 at residue 27: differences in the spatial and temporal behavior of phospho-Cav-2 (pY19 and pY27)." in: Biochemistry, Vol. 43, Issue 43, pp. 13694-706, 2004 (PubMed).
Zaas, Duncan, Li et al.: "Pseudomonas invasion of type I pneumocytes is dependent on the expression and phosphorylation of caveolin-2." in: The Journal of biological chemistry, Vol. 280, Issue 6, pp. 4864-72, 2005 (PubMed).
|Hosts||Rabbit (64), Goat (1)|
|Reactivities||Human (45), Mouse (Murine) (37), Rat (Rattus) (37), Cow (Bovine) (2), Monkey (1), Pig (Porcine) (1)|
|Applications||Western Blotting (WB) (34), Immunofluorescence (IF) (30), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunoprecipitation (IP) (5), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunohistochemistry (IHC) (4), Immunoelectron Microscopy (IEM) (2), Flow Cytometry (FACS) (1)|
|Conjugates||Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)|
|Epitopes||pTyr19 (17), pSer23 (3), pTyr27,N-Term (3), N-Term (2), pSer36 (2), pTyr27 (2), Center (1)|