Caveolin 2 (CAV2) (pTyr27) antibody

Antigen: Caveolin 2 (CAV2)

Synonyms: CAV, MGC12294, AI447843, MGC72322, Cav-2, CAV2, CAV-2, zgc:86696, wu:fc52e01, Caveolin-2, cav, MGC108281, caveolin-2, cav2, cav-2

Binding Site: pTyr27

Clonality: Monoclonal (40)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Fluorescence Microscopy (FM)

Catalog no.: ABIN967595

Additional Information

Alternative name: Caveolin 2

Immunogen: Phosphorylated Human Caveolin 2

Format: Liquid

Isotype: IgG1, kappa

Clone: 40

Description: The caveolins are a family of transmembrane proteins that are required for the structural integrity and functions of non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. The intracellular functions of caveolae include roles in cholesterol homeostasis, signal transduction, and vesicle transport. Caveolins 1 and 2 form hetero-oligomers in the endoplasmic reticulum, Golgi complex and trans-Golgi-derived transport vesicles in most tissues. The highest levels of these complexes are found in adipocytes, fibroblasts, and endothelial, epithelial, and smooth muscle cells. Upon phosphorylation at tyrosine 19 or 27, caveolin 2 dissociates from caveolin 1, and the two phosphorylated forms of caveolin 2 localize to distinct areas of the cell. There are reports that internalization of caveolae may be regulated by tyrosine phosphorylation of caveolin 2.
The 40/Caveolin 2 monoclonal antibody recognizes the phosphorylated tyrosine 27 (pY27) of caveolin 2.

Characteristics: 1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 20 kdA

Application Details

Concentration: 0.25 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: The antibody should be kept at -20°C for long term storage, and it may be kept at 4°C for short term storage.

Research Area: Cardiovascular, Atherosclerosis, Organelles

Restrictions: For Research Use only

Images

Western blot analysis of Caveolin 2 (pY27) in human endothelial cells. Lysates from pervanadate-stimulated endothelial cells (ABIN968630) were either treated with 50 µg/ml alkaline phosphatase (ICN Biomedicals) for 30 minutes at 37°C (left lane) or untreated (right lane), then probed with purified 40/Caveolin 2 mAb at 0.5 µg/ml. Caveolin 2 (pY27) is identified as a band of 20 kDa in the untreated lysate.

Caveolin 2 (pY27) staining on tonsil. Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (first panel) or treated with a phosphatase to eliminate all phosphorylation (second panel). The tissue sections were stained with purified Mouse anti-Caveolin 2 (pY27) with Hematoxylin counterstaining. Original magnification: 20X.

Publications

Product: Conner, Schmid: "Regulated portals of entry into the cell." in: Nature, Vol. 422, Issue 6927, pp. 37-44, 2003 (PubMed).

Williams, Lisanti: "The caveolin proteins." in: Genome biology, Vol. 5, Issue 3, pp. 214, 2004 (PubMed).

Wang, Lee, Capozza et al.: "Tyrosine phosphorylation of caveolin-2 at residue 27: differences in the spatial and temporal behavior of phospho-Cav-2 (pY19 and pY27)." in: Biochemistry, Vol. 43, Issue 43, pp. 13694-706, 2004 (PubMed).

Zaas, Duncan, Li et al.: "Pseudomonas invasion of type I pneumocytes is dependent on the expression and phosphorylation of caveolin-2." in: The Journal of biological chemistry, Vol. 280, Issue 6, pp. 4864-72, 2005 (PubMed).