MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pSer298)

Details for Product anti-MAP2K1 Antibody No. ABIN967599, Supplier: Log in to see
Antigen
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • Map2k1
  • MAP2K1
  • map2k1
  • MEK1
Alternatives
anti-Human MAP2K1 antibody for Immunofluorescence (fixed cells)
Epitope
pSer298
37
37
34
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28
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24
19
15
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1
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Reactivity
Human, Mouse (Murine)
633
426
375
108
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45
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26
22
21
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8
5
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4
2
1
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Host
Mouse
504
128
5
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2
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Clonality (Clone)
Monoclonal ()
Conjugate
This MAP2K1 antibody is un-conjugated
16
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11
9
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8
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6
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2
2
Application
Western Blotting (WB)
495
197
161
133
121
78
27
22
16
10
9
6
6
6
2
2
2
1
1
1
1
Options
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Brand BD Pharmingen™
Immunogen Phosphorylated Human MEK1 Peptide
Clone J114-64
Isotype IgG1, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MEK1 (MAP2K1 Antibody Abstract)
Background MEK (Map/Erk Kinase) 1 and 2 are serine/threonine kinases, also known as MAP kinase kinases (MAP2K1 and 2, MAPKK1 and 2, or MKK1 and 2). They activate the MAP (Mitogen-Activated Protein) kinases, also known as ERKs (Extracellular signal Regulated Kinases), which are critical kinases in multiple signal transduction pathways that regulate cell growth and differentiation. Activation of MEK 1 and 2 is dependent upon phosphorylation of serines 218 and/or 222 by activated MAP kinase kinase kinases (MAP3Ks), such as the Raf isoforms. Hormones, growth and differentiating factors, or tumor promoters induce Raf activation via activation of Ras proteins. Alternatively, cellular adhesion can lead to phosphorylation of MEK1 at serine 298 (S298), mediated by p21-activated kinase (PAK). The S298-phosphorylated MEK1 has an enhanced capacity to interact with Raf, resulting in MEK1 activation. The J114-64 monoclonal antibody recognizes the phosphorylated S298 of MEK1.
Molecular Weight 45 kDa
Research Area Signaling, Tyrosine Kinases
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pSer298) (ABIN967599) Western blot analysis of MEK1 (pS298) in mouse embryonic fibroblasts. Lysates from de...
Product cited in: Slack-Davis, Eblen, Zecevic, Boerner, Tarcsafalvi, Diaz, Marshall, Weber, Parsons, Catling: "PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation." in: The Journal of cell biology, Vol. 162, Issue 2, pp. 281-91, 2003 (PubMed).

Eblen, Slack, Weber, Catling: "Rac-PAK signaling stimulates extracellular signal-regulated kinase (ERK) activation by regulating formation of MEK1-ERK complexes." in: Molecular and cellular biology, Vol. 22, Issue 17, pp. 6023-33, 2002 (PubMed).

Kolch: "Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions." in: The Biochemical journal, Vol. 351 Pt 2, Issue 2, pp. 289-305, 2001 (PubMed).