p65 antibody (Nuclear Factor-KB P65) (pSer536)

Details for Product anti-NFkBP65 Antibody No. ABIN967600, Supplier: Log in to see
Antigen
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Alternatives
anti-Human p65 antibody for BioImaging
Epitope
pSer536
137
70
66
56
51
45
43
40
38
35
33
31
24
21
18
17
16
15
15
13
13
13
12
11
10
9
9
8
7
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1179
678
520
73
59
55
31
30
28
25
15
8
8
6
5
4
2
1
1
Host
Mouse
1012
144
17
15
11
1
Clonality (Clone)
Monoclonal ()
Conjugate
This p65 antibody is un-conjugated
34
34
26
21
19
18
16
16
13
13
13
9
9
9
9
9
9
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Application
Western Blotting (WB)
858
428
348
250
214
149
118
87
84
28
22
18
17
11
11
6
6
6
5
4
4
2
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Brand BD Pharmingen™
Immunogen Phosphorylated Human or Mouse NF-kappaB p65 Peptide
Clone J144-460
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name NF-kappa B p65 (NFkBP65 Antibody Abstract)
Background Nuclear factor kB (NF-kB) is a ubiquitously expressed transcription factor that regulates the expression of 200-300 genes. It is crucial for basic cellular responses to stress and pathogens, such as proliferation, survival, development, and apoptosis. The most studied NF-kB complex consists of the p50 (also known as NF-kB1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor IkB and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkB. This complex prevents nuclear translocation and activity of NF-kB. In response to stimuli such as cytokines, LPS, DNA damage, and viral infections, IkB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkB/NF-kB complex, allowing the free heterodimeric NF-kB to translocate to the nucleus. Furthermore, optimal activation of NF-kB requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF-kB dimers bind to the kB sites within promoters and enhancers and function as transcriptional activators. The J144-460 monoclonal antibody recognizes the phosphorylated serine 536 (pS536) in the transactivation domain of human NF-kB p65.
Molecular Weight 65 kDa
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-p65 antibody (Nuclear Factor-KB P65) (pSer536) (ABIN967600) Western blot analysis of NF-kappaB p65 (pS536) in transformed human epithelioid carci...
Product cited in: Natoli, Saccani, Bosisio, Marazzi: "Interactions of NF-kappaB with chromatin: the art of being at the right place at the right time." in: Nature immunology, Vol. 6, Issue 5, pp. 439-45, 2005 (PubMed).

Siebenlist, Brown, Claudio: "Control of lymphocyte development by nuclear factor-kappaB." in: Nature reviews. Immunology, Vol. 5, Issue 6, pp. 435-45, 2005 (PubMed).

Viatour, Merville, Bours, Chariot: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, 2005 (PubMed).

Did you look for something else?