Images for product: anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody
Western blot analysis of EGF Receptor (pY1173) in human epidermis (left figure). Lysates from control (first panel) and EGF-treated (second panel) human A-431 epidermoid carcinoma were probed with the purified mouse anti-EGF Receptor (pY1173) monoclonal antibody at concentrations of 0.4 µg/mL, 0.2 µg/mL, and 0.1 µg/mL (lanes 1, 2, and 3, respectively). EGF Receptor (pY1173) is identified as a band of 175 kDa in the treated cells.
Immunohistochemical analysis for EGF Receptor (pY1173) staining on tonsil (right figure). Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (first panel) or treated with a phosphatase to eliminate all phosphorylation (second panel). The tissue sections were stained with purified Mouse anti-EGF Receptor (pY1173) with Hematoxylin counterstaining. No staining was seen on sections of unstimulated tonsil (data not shown). Original magnification: 20X.