Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody

Details for Product No. ABIN967603
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Antigen
Synonyms
ERBB, HER1, mENA, ERBB1, PIG61, 9030024J15Rik, AI552599, Erbb, Errb1, Errp, Wa5, wa-2, wa2, ErbB-1, c-erbB, C-erb, CG10079, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top ... show more
ERBB, HER1, mENA, ERBB1, PIG61, 9030024J15Rik, AI552599, Erbb, Errb1, Errp, Wa5, wa-2, wa2, ErbB-1, c-erbB, C-erb, CG10079, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DER1, DEgfr, Degfr, Der, DmHD-33, Dmel\\CG10079, EFG-R, EGF-R, EGFR, EGFr, EGfr, EK2-6, Egf, Egf-r, EgfR, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, TOP, Torpedo/DER, Torpedo/Egfr, d-egf-r, dEGFR, dEGFR1, dEgfr, der, egfr, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, mor1, top, top/DER, top/flb, torpedo/Egfr, torpedo/egfr, EGFR12, EGFR15, egfr1, Erbb2 show less
Epitope
pTyr1173
(66), (50), (48), (45), (44), (41), (39), (39), (38), (32), (28), (25), (23), (17), (17), (17), (16), (14), (14), (13), (13), (13), (13), (13), (12), (12), (12), (11), (11), (9), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(1252), (663), (617), (163), (147), (125), (79), (5), (5), (1), (1)
Host
Mouse
(1001), (234), (22), (13), (4), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(60), (32), (27), (25), (25), (25), (25), (25), (25), (25), (25), (5), (2), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), ELISA
(839), (390), (288), (250), (201), (149), (111), (101), (74), (58), (29), (16), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN967603
Quantity 0.1 mg
Price
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Immunogen Phosphorylated Human EGF Receptor Peptide
Clone 9H2
Isotype IgG1, kappa
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name EGF Receptor
Background Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF Receptor (EGFR) binding and activation of intrinsic tyrosine kinase activity. EGFR, also known as ErbB1 or HER1, is a member of the ErbB class of receptor protein tyrosine kinases. It has an extracellular ligand-binding domain, a single transmembrane region, and a cytoplasmic region containing a protein tyrosine kinase domain and a c-terminal regulatory domain with many phosphorylation sites. Following ligand binding, EGFR forms homodimers and heterodimers with ErbB2. Specific C-terminal tyrosine residues are then autophosphorylated and, in turn, bind to adaptor proteins, kinases, or protein tyrosine phosphatases. Specifically, phosphorylated tyrosine 1173 (Y1173) interacts with Shc, SHP1, and PLCg, which mediate downstream signaling cascades and negative feedback regulation of EGFR activation. Inappropriate expression or mutations of EGFR and/or deregulation of its signaling pathways are associated with many types of cancer, making EGFR a promising target for cancer therapies.
The 9H2 monoclonal antibody recognizes the phosphorylated Y1173 in the regulatory domain of human EGFR.
Molecular Weight 175 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody Western blot analysis of EGF Receptor (pY1173) in human epidermis (left figure). Lysates from control (first panel) and EGF-treated (second panel) human A-431 epidermoid carcinoma were probed with the purified mouse anti-EGF Receptor (pY1173) monoclonal antibody at concentrations of 0.4 µg/mL, 0.2 µg/mL, and 0.1 µg/mL (lanes 1, 2, and 3, respectively). EGF Receptor (pY1173) is identified as a band of 175 kDa in the treated cells.
anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (2) Immunohistochemical analysis for EGF Receptor (pY1173) staining on tonsil (right figure). Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (first panel) or treated with a phosphatase to eliminate all phosphorylation (second panel). The tissue sections were stained with purified Mouse anti-EGF Receptor (pY1173) with Hematoxylin counterstaining. No staining was seen on sections of unstimulated tonsil (data not shown). Original magnification: 20X.
Product cited in: Olayioye, Neve, Lane et al.: "The ErbB signaling network: receptor heterodimerization in development and cancer." in: The EMBO journal, Vol. 19, Issue 13, pp. 3159-67, 2000 (PubMed).

Mendelsohn, Baselga: "Status of epidermal growth factor receptor antagonists in the biology and treatment of cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 21, Issue 14, pp. 2787-99, 2003 (PubMed).

Hosts (1001), (234), (22), (13), (4), (1), (1)
Reactivities (1252), (663), (617), (163), (147), (125), (79), (5), (5), (1), (1)
Applications (839), (390), (288), (250), (201), (149), (111), (101), (74), (58), (29), (16), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Conjugates (60), (32), (27), (25), (25), (25), (25), (25), (25), (25), (25), (5), (2), (1), (1), (1), (1)
Epitopes (66), (50), (48), (45), (44), (41), (39), (39), (38), (32), (28), (25), (23), (17), (17), (17), (16), (14), (14), (13), (13), (13), (13), (13), (12), (12), (12), (11), (11), (9), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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