Retinoblastoma 1 antibody (RB1) (pSer780)

Details for Product anti-RB1 Antibody No. ABIN967604, Supplier: Log in to see
Antigen
  • RB1
  • OSRC
  • RB
  • p105-Rb
  • pRb
  • pp110
  • Rb
  • Rb-1
  • zgc:154147
  • P105-RB
  • PP110
  • RB11
  • retinoblastoma 1 (including osteosarcoma)
  • retinoblastoma 1
  • Retinoblastoma-associated protein
  • RB1
  • rb
  • Rb1
  • rb1
Alternatives
anti-Human Retinoblastoma 1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
pSer780
54
54
53
43
37
29
29
20
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18
15
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14
8
8
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7
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3
3
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2
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2
2
1
1
1
1
1
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1
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1
1
1
1
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1
1
1
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1
1
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1
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Reactivity
Human
634
343
237
10
9
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5
5
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4
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3
2
1
1
Host
Mouse
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1
Clonality (Clone)
Monoclonal ()
Conjugate
This Retinoblastoma 1 antibody is un-conjugated
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26
16
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7
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7
5
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5
5
5
3
2
1
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Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
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241
171
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94
93
50
41
30
24
10
9
7
7
6
1
1
1
1
Options
Supplier
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Phosphorylated Human Rb
Clone J146-35
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Rb (RB1 Antibody Abstract)
Background The retinoblastoma gene product (Rb) is well known as a tumor suppressor and is either absent or mutated in many human tumors. Retrovirus-mediated gene transfer of the wild-type Rb gene into several Rb mutant neoplastic cell lines suppresses their tumorgenicity. Rb is a 110-kDa nuclear phosphoprotein that undergoes differential phosphorylation during the cell cycle. During G1 phase, Rb is predominantly in a hypophosphorylated state. It becomes increasingly phosphorylated throughout the cell cycle until late mitosis, when substantial dephosphorylation occurs. Hypophosphorylated Rb interacts with a number of cellular proteins including the E2F transcription factor, several cyclins, RBP-1, RBP-2, c-Abl, c-myc, N-myc, and p46. Phosphorylation of Rb at various sites, by Cyclin-dependent protein kinases, inhibits the binding of Rb to these proteins. Rb is thought to mediate its effects, in part, via the repression of genes required for proliferation. For example, Rb is specifically recruited to promoters containing E2F sites and actively represses E2F mediated transcription. Rb also stimulates the activity of other transcription factors, although the mechanisms are less clearly defined. Thus, Rb appears to regulate transcription in its aim to control cell growth.
The J146-35 monoclonal antibody recognizes Rb phosphorylated at serine 780 (pS780), which affects Rb binding to E2F. The orthologous phosphorylation sites in mouse and rat Rb are serines 773 and 751, respectively.
Molecular Weight 110 kDa
Research Area Cancer, Transcription Factors
Pathways Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-Retinoblastoma 1 (RB1) (pSer780) antibody (ABIN967604) Western blot analysis of Rb (pS780) in human embryonic skin cells. Lysates from serum...
 image for anti-Retinoblastoma 1 (RB1) (pSer780) antibody (ABIN967604) Rb (pS780) staining on tonsil. Fresh human tonsil, stimulated with in 5 mM Pervanadat...
Western Blotting (WB) image for anti-Retinoblastoma 1 (RB1) (pSer780) antibody (ABIN967604) anti-Retinoblastoma 1 (RB1) (pSer780) antibody (Image 3)
Product cited in: Cobrinik: "Pocket proteins and cell cycle control." in: Oncogene, Vol. 24, Issue 17, pp. 2796-809, 2005 (PubMed).

Knudsen, Wang: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, 1997 (PubMed).

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