Images for product: anti-Retinoblastoma 1 (RB1) (pSer780) antibody
Western blot analysis of Rb (pS780) in human embryonic skin cells. Lysates from serum-starved (first panel) and fetal bovine serum-stimulated (second panel) WS1 cell line were probed with purified mouse anti-Rb (pS780) monoclonal antibody at concentrations of 4.0 (lanes 1 and 4), 2.0 (lanes 2 and 5), and 1.0 µg/ml (lanes 3 and 6). Rb (pS780) is identified as a band of 110 kDa in the stimulated cells.
Rb (pS780) staining on tonsil. Fresh human tonsil, stimulated with in 5 mM Pervanadate solution for 2 hours (top row) or unstimulated (bottom row), was fixed in formalin and processed. Following antigen retrieval with BD Retrievagen A buffer, the sections were either left untreated (left column) or treated with a phosphatase to eliminate all phosphorylation (right column). The tissue sections were stained with purified Mouse anti-Rb (pS780) monoclonal antibody with Hematoxylin counterstaining. Original magnification: 20X.