V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (pTyr207) antibody
|Synonyms||Crkol, AA589403, AI325100, 1110025F07Rik, CRKL, MGC63469, zgc:63469, wu:fa55g05, wu:fj60e01|
Alternatives Western Blotting (WB)
|3 references available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Phosphorylated Human, Mouse, and Rat CrkL Peptide|
|Description||Crk-Like (CrkL) is an adaptor protein that is preferentially expressed in hematopoietic cells and is encoded by a gene that is homologous to the viral oncogene v-crk (chicken tumor virus no. 10 regulator of kinase). Its SH2 and SH3 domains bind to a variety of effector proteins, such as paxillin, p130Cas, c-Cbl, c-Abl, and C3G. These interactions are involved in the regulation of cellular migration, adhesion, and transformation. Tyrosine 207 (Y207) of CrkL is phosphorylated in activated hematopoietic cells and in chronic myelogenous leukemia. This site may be a negative regulator of protein complex formation and biological activity. The K30-391.11.30 monoclonal antibody recognizes the phosphorylated Y207 of human CrkL.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||39 kDa|
Related Products: ABIN967389, ABIN968587
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Research Area||Cancer, Transcription Factors|
|Restrictions||For Research Use only|
Senechal, Heaney, Druker et al.: "Structural requirements for function of the Crkl adapter protein in fibroblasts and hematopoietic cells." in: Molecular and cellular biology, Vol. 18, Issue 9, pp. 5082-90, 1998 (PubMed).
Arai, Nosaka, Kohsaka et al.: "CrkL activates integrin-mediated hematopoietic cell adhesion through the guanine nucleotide exchange factor C3G." in: Blood, Vol. 93, Issue 11, pp. 3713-22, 1999 (PubMed).
Feller: "Crk family adaptors-signalling complex formation and biological roles." in: Oncogene, Vol. 20, Issue 44, pp. 6348-71, 2001 (PubMed).