V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (pTyr207) antibody

Details for Product No. ABIN967605
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Antigen
Synonyms Crkol, AA589403, AI325100, 1110025F07Rik, CRKL, wu:fa55g05, wu:fj60e01, zgc:63469
Epitope
pTyr207
(24), (12), (8), (7), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(106), (65), (60), (36), (25), (24)
Host
Mouse
(82), (19), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB)
(78), (46), (30), (23), (20), (16), (8), (6), (4), (3), (2), (2), (2), (1), (1)
Pubmed 3 references available
Catalog no. ABIN967605
Quantity 0.1 mg
Price
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Immunogen Phosphorylated Human, Mouse, and Rat CrkL Peptide
Clone K30-391-11-30
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name CrkL
Background Crk-Like (CrkL) is an adaptor protein that is preferentially expressed in hematopoietic cells and is encoded by a gene that is homologous to the viral oncogene v-crk (chicken tumor virus no. 10 regulator of kinase). Its SH2 and SH3 domains bind to a variety of effector proteins, such as paxillin, p130Cas, c-Cbl, c-Abl, and C3G. These interactions are involved in the regulation of cellular migration, adhesion, and transformation. Tyrosine 207 (Y207) of CrkL is phosphorylated in activated hematopoietic cells and in chronic myelogenous leukemia. This site may be a negative regulator of protein complex formation and biological activity. The K30-391.11.30 monoclonal antibody recognizes the phosphorylated Y207 of human CrkL.
Molecular Weight 39 kDa
Research Area Cancer, Transcription Factors
Comment

Related Products: ABIN967389, ABIN968587

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (pTyr207) antibody Western blot analysis of CrkL (pY207) in human chronic myelogenous leukemia. Lysates from the K-562 cell line (ABIN968586) were probed with purified mouse anti-CrkL (pY207) monoclonal antibody at concentrations of 0.0125 (lanes 1 and 4), 0.00625 (lanes 2 and 5), and 0.00312 µg/ml (lanes 3 and 6) with (lanes 1-3) or without (lanes 4-6) lambda protein phosphatase treatment. CrkL (pY207) is identified as a band of 39 kDa in the untreated cells.
Product cited in: Senechal, Heaney, Druker et al.: "Structural requirements for function of the Crkl adapter protein in fibroblasts and hematopoietic cells." in: Molecular and cellular biology, Vol. 18, Issue 9, pp. 5082-90, 1998 (PubMed).

Arai, Nosaka, Kohsaka et al.: "CrkL activates integrin-mediated hematopoietic cell adhesion through the guanine nucleotide exchange factor C3G." in: Blood, Vol. 93, Issue 11, pp. 3713-22, 1999 (PubMed).

Feller: "Crk family adaptors-signalling complex formation and biological roles." in: Oncogene, Vol. 20, Issue 44, pp. 6348-71, 2001 (PubMed).

Hosts (82), (19), (10)
Reactivities (106), (65), (60), (36), (25), (24)
Applications (78), (46), (30), (23), (20), (16), (8), (6), (4), (3), (2), (2), (2), (1), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (24), (12), (8), (7), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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