Catenin (Cadherin-Associated Protein), delta 1 (CTNND1) (pThr916) antibody

Antigen: Catenin (Cadherin-Associated Protein), delta 1 (CTNND1)

Synonyms: CAS, p120, CTNND, P120CAS, P120CTN, KIAA0384, CTNND1, cas, Xp120, ctnnd, p120cas, p120ctn, p120(ctn), Xp120(ctn), p120-catenin, DKFZp469D181

Binding Site: pThr916

Clonality: Monoclonal (1)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN967615

Additional Information

Alternative name: p120 Catenin

Immunogen: Phosphorylated Human p120 Catenin peptide

Format: Liquid

Isotype: IgG2b, kappa

Clone: 1

Description: The membrane associated protein pp120 Src substrate (p120 catenin, p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src-transformed cells. It shares structural similarity with the Drosophila Armadillo protein and the vertebrate b-catenin and g-catenin proteins in its 42-amino acid Arm domain. p120 catenin is localized to the E-Cadherin/catenin cell adhesion complex. Like b- and g-catenin, p120 catenin directly associates with the cytoplasmic C-terminus of E-Cadherin via its Arm domain. It exists as four isoforms that range in size from 90 to 115 kDa. Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered expression contributes to malignancy. Phosphorylation of multiple serine (S252, S268, S288, and S879), and threonine (T310 and T916) residues in p120 catenin may regulate its activity. The S879 residue is phosphorylated after PKC activation, while the S268 site is dephosphorylated after PKC activation. The latter residue is phosphorylated in vitro by p160 Rock. S252 and T310 residues are phosphorylated in vitro by GSK3b. Thus, p120 catenin function may be regulated in a complex manner through both serine and threonine phosphorylation.
The 1/Catenin monoclonal antibody recognizes the phosphorylated T916 in the carboxy-terminal tail of human p120 catenin. The orthologous phosphorylation site in mouse p120 catenin is T889.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 120 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Western blot analysis of p120 catenin (pT916) in human epidermis. Lysates from control (first panel) and calyculin A-plus-okadaic acid-treated (second panel) human A-431 epidermoid carcinoma were probed with purified mouse anti-p120 catenin (pT916) monoclonal antibody at concentrations of 0.0039 µg/ml (lanes 1,4), 0.0019 µg/ml (lanes 2,5), and 0.001 µg/ml (lanes 3,6). p120 catenin (pT916) is identified as a band of 120 kDa in the treated cells.

Publications

Product: Reynolds, Roczniak-Ferguson: "Emerging roles for p120-catenin in cell adhesion and cancer." in: Oncogene, Vol. 23, Issue 48, pp. 7947-56, 2004 (PubMed).