Catenin (Cadherin-Associated Protein), delta 1 (CTNND1) (pThr916) antibody
|Synonyms||CAS, p120, CTNND, P120CAS, P120CTN, KIAA0384, CTNND1, cas, Xp120, ctnnd, p120cas, p120ctn, p120(ctn), Xp120(ctn), p120-catenin, DKFZp469D181|
Alternatives Western Blotting (WB)
|1 reference available|
|Quantity||0.1 mg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Alternative name||p120 Catenin|
|Immunogen||Phosphorylated Human p120 Catenin peptide|
The membrane associated protein pp120 Src substrate (p120 catenin, p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src-transformed cells. It shares structural similarity with the Drosophila Armadillo protein and the vertebrate b-catenin and g-catenin proteins in its 42-amino acid Arm domain. p120 catenin is localized to the E-Cadherin/catenin cell adhesion complex. Like b- and g-catenin, p120 catenin directly associates with the cytoplasmic C-terminus of E-Cadherin via its Arm domain. It exists as four isoforms that range in size from 90 to 115 kDa. Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered expression contributes to malignancy. Phosphorylation of multiple serine (S252, S268, S288, and S879), and threonine (T310 and T916) residues in p120 catenin may regulate its activity. The S879 residue is phosphorylated after PKC activation, while the S268 site is dephosphorylated after PKC activation. The latter residue is phosphorylated in vitro by p160 Rock. S252 and T310 residues are phosphorylated in vitro by GSK3b. Thus, p120 catenin function may be regulated in a complex manner through both serine and threonine phosphorylation.
The 1/Catenin monoclonal antibody recognizes the phosphorylated T916 in the carboxy-terminal tail of human p120 catenin. The orthologous phosphorylation site in mouse p120 catenin is T889.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||120 kDa|
Related Products: ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4°C.|
|Restrictions||For Research Use only|
Reynolds, Roczniak-Ferguson: "Emerging roles for p120-catenin in cell adhesion and cancer." in: Oncogene, Vol. 23, Issue 48, pp. 7947-56, 2004 (PubMed).
|Hosts||Rabbit (35), Mouse (10)|
|Reactivities||Human (43), Mouse (Murine) (12), Rat (Rattus) (12), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||Western Blotting (WB) (35), ELISA (13), Immunoprecipitation (IP) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunocytochemistry (ICC) (10), Immunohistochemistry (IHC) (10), Immunofluorescence (IF) (6), Flow Cytometry (FACS) (4), Dot Blot (Dot) (1)|
|Epitopes||C-Term (3), N-Term (3), pTyr228 (3), pTyr96 (2), AA 625-675 (1), Internal Region (1), Ser228 (1), Ser280 (1), pSer268 (1), pThr310 (1), pThr916 (1), pTyr280 (1)|