Images for product: anti-Transportin (N-Term) antibody
Western blot analysis of transportin in human T leukemia. Jurkat cell lysate (ABIN968537) was probed with Mouse anti-transportin monoclonal antibody at concentrations of 0. 125 (lane 1), 0.0625 (lane 2), and 0.03125 myg/ml (lane 3). Transportin is identified as a band of 95 - 101 kDa.
Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96-well imaging plate at ~10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the Mouse anti-Transportin monoclonal antibody. The second-step reagent was Alexa Fluor® 555 goat anti-mouse Ig (Invitrogen). Images were captured on a BD Pathway™ 855 Bioimager using a 20x objective. This antibody also stained A549 (ATCC CCL-185) and U-2 OS (ATCC HTB-96) cells and worked with both the Triton™ X-100 and alcohol perm protocols.