Tumor Protein P73 (TP73) (AA 380-367) antibody

Details for Product No. ABIN967628
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Antigen
Synonyms P73, TP73, tp73, Tp73, p73, Trp73, TAp73, zp73theta
Epitope
AA 380-367
(38), (13), (10), (7), (5), (5), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(158), (68), (46), (8), (2)
Host
Mouse
(139), (26)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Immunoprecipitation (IP), Western Blotting (WB)
(142), (35), (35), (30), (26), (20), (20), (8), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN967628
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Immunogen Human p73alpha:GST fusion protein
Clone ER-15
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name p73
Background P53 is a tumor suppressor which acts as an S-phase checkpoint for DNA damage. The gene for p53 is the most commonly mutated gene identified in human cancers. Recently, a new member of the p53 family, p73, has been identified. p73 is structurally homologous to p53 in several regions, including the p53 N-terminal transactivation domain and C-terminal oligomerization domains, as well as the region corresponding to the p53 DNA-binding domain. p73, when overexpressed, can promote p53-like functions, including induction of apoptosis and induction of transcription from p53-responsive promoters such as p21. Despite structural and apparent functional homology, data suggests that these proteins may have distinct functions as well. For example, viral oncoproteins such as Adenovirus E1B 55k and HPV E6, which bind to and thus inactivate p53 during the process of transformation, do not bind to p73. In addition, unlike p53, p73 expression is not induced by DNA damage, i.e., UV irradiation. Several p73 splice variants have been identified, including alpha (full length), beta (missing exon 13), gamma (missing exon 11) and delta (missing exons 11, 12, and 13). Two hybrid analysis has shown variable interaction(s) between these isoforms in vitro. Many types of normal, tumor and virally-transformed cell lines express detectable levels of p73, however, the relative expression of p73 isoforms, as well as their functional activity, appears to be differentially regulated in various cell types. p73 alpha and beta isoforms migrate at molecular weights of 80 kD (alpha), 70 kD (beta), respectively. The ER-15 antibody reacts with human p73alpha and beta. This antibody is routinely tested by western blot analysis.
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA
Application Notes For western blot analysis use 0.5-2 µg/ml.
Human cell lines including 293 adenovirus-transformed kidney cells (ATCC CRL-1573) and cos-7 SV-40 transformed monkey kidney (ATCC CRL-1651) cell lines are recommended as positive controls.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Tumor Protein P73 (TP73) (AA 380-367) antibody Western blot analysis of p73. Lysate from 293 human embryonic kidney cells was probed with anti-p73 (clone ER-15, ABIN967628). Clone ER-15 identifies p73.
anti-Tumor Protein P73 (TP73) (AA 380-367) antibody (2) anti-Tumor Protein P73 (TP73) (AA 380-367) antibody (Image 2)
Product cited in: Kaghad, Bonnet, Yang et al.: "Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers." in: Cell, Vol. 90, Issue 4, pp. 809-19, 1997 (PubMed).

Jost, Marin, Kaelin: "p73 is a simian [correction of human] p53-related protein that can induce apoptosis." in: Nature, Vol. 389, Issue 6647, pp. 191-4, 1997 (PubMed).

Marin, Jost, Irwin et al.: "Viral oncoproteins discriminate between p53 and the p53 homolog p73." in: Molecular and cellular biology, Vol. 18, Issue 11, pp. 6316-24, 1998 (PubMed).

De Laurenzi, Costanzo, Barcaroli et al.: "Two new p73 splice variants, gamma and delta, with different transcriptional activity." in: The Journal of experimental medicine, Vol. 188, Issue 9, pp. 1763-8, 1998 (PubMed).

Zhu, Jiang, Zhou et al.: "The potential tumor suppressor p73 differentially regulates cellular p53 target genes." in: Cancer research, Vol. 58, Issue 22, pp. 5061-5, 1998 (PubMed).

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