Cyclin-Dependent Kinase 2 (CDK2) (AA 287-298) antibody

Details for Product No. ABIN967634
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Antigen
Synonyms MGC84068, cinp, CINP, p33(CDK2), A630093N05Rik, wu:fa10c02, zgc:56598, zgc:77684, eg1, CDKN2, cdk2L
Epitope
AA 287-298
(42), (36), (17), (11), (8), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(204), (112), (103), (28), (17), (12), (12), (5), (4), (4), (3), (2), (2), (1)
Host
Rabbit
(186), (31), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP)
(187), (74), (51), (51), (40), (22), (20), (18), (17), (17), (10), (8), (4), (1), (1), (1), (1), (1)
Pubmed 6 references available
Quantity 0.1 mL
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Catalog No. ABIN967634
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Immunogen Human cdk2 synthetic peptide
Isotype Ig
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from antiserum by negative adsorption and affinity chromatography.
Alternative Name Cdk2
Background Cyclins and cyclin-dependent kinases (cdks) are evolutionarily conserved proteins that are essential for cell cycle control in eukaryotes. Cdks are catalytic subunits whose activity requires interaction with their regulatory subunits, the cyclins, as well as specific phosphorylation events. The precise timing of cyclin-cdk activity during the cell cycle determines whether the cell cycle continues or becomes blocked. Cdks 2, 4, 5, and 6 may associate with D-type cyclins. Interaction between cdk2 and cyclin E during G1/S transition creates a complex with histone H1 kinase activity. This complex is thought to be required for the initiation and progression of DNA replication during S phase. Cdk2-cyclin A complexes appear during late S phase and also play a role in progression of DNA replication. Substrates for cdk-cyclin complexes include nuclear lamins, histones, oncogenes, (c-src, c-abl, SV40 large-T), tumor suppressor genes (e.g., RB and p53), nucleolin, RNA polymerase II and others. Cdk2 migrates at ~33 kDa on SDS-PAGE. The polyclonal antibodies recognize human and mouse cdk2. The antibodies do not react with other known cdk proteins. A synthetic peptide corresponding to amino acids 287-298 (QDVTKPVPHLRL) at the C-terminus of human cdk2, with the addition of a C-terminal cysteine residue (C) to facilitate coupling to KLH, was used as immunogen.
The polyclonal antibodies recognize human and mouse cdk2. The antibodies do not react with other known cdk proteins. A synthetic peptide corresponding to amino acids 287-298 (QDVTKPVPHLRL) at the C-terminus of human cdk2, with the addition of a C-terminal cysteine residue (C) to facilitate coupling to KLH, was used as immunogen.
Molecular Weight 33 kDa
Research Area Cancer, Cell Cycle, Kinases/Phosphatases
Application Notes Applications include western blot analysis (1:1000). Human cell lines including HeLa cervical carcinoma cells (ATCC CCL-2), 293 embryonic kidney (ATCC CRL-1673), and WI-38 lung fibroblasts (ATCC CCL-75) are suggested as positive controls for these applications.
Comment

Related Products: ABIN967390, ABIN968535

Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 287-298) antibody Western blot analysis of Cdk2. Lysate from HeLa human cervical carcinoma cells was probed with anti-CDK2 (ABIN967634). Cdk2 is identified at ~33 kDa.
anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 287-298) antibody (2) anti-Cyclin-Dependent Kinase 2 (CDK2) (AA 287-298) antibody (Image 2)
Product cited in: Zhang, Xiong, Beach: "Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes." in: Molecular biology of the cell, Vol. 4, Issue 9, pp. 897-906, 1994 (PubMed).

Peter, Herskowitz: "Joining the complex: cyclin-dependent kinase inhibitory proteins and the cell cycle." in: Cell, Vol. 79, Issue 2, pp. 181-4, 1994 (PubMed).

Sherr: "G1 phase progression: cycling on cue." in: Cell, Vol. 79, Issue 4, pp. 551-5, 1994 (PubMed).

Heichman, Roberts: "Rules to replicate by." in: Cell, Vol. 79, Issue 4, pp. 557-62, 1994 (PubMed).

Hunter, Pines: "Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age." in: Cell, Vol. 79, Issue 4, pp. 573-82, 1994 (PubMed).

Xiong, Zhang, Beach: "Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation." in: Genes & development, Vol. 7, Issue 8, pp. 1572-83, 1993 (PubMed).

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