Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B) (AA 184-197) antibody

Antigen: Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B)

Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B

Binding Site: AA 184-197

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967635

Additional Information

Alternative name: p27 Kip1

Immunogen: Mouse p27 peptide

Format: Liquid

Description: P27 (Kip1) is a cyclin-dependent kinase inhibitor (cdkI) that associates with cyclin/cdk complexes to inhibit their catalytic activity. p27 was first identified as a result of its role in TGF-beta-induced G1 phase arrest and cell-cell contact. In vitro, p27 binds tightly to cyclin D-Cdk4, cyclin E-Cdk2, and cyclin A-Cdk2 complexes to inhibit their activity. In non-dividing cells, the level of p27 is elevated and its activity gradually decreases as cells reach S phase. Mitogenic stimulation causes the cyclin D-CDK complex to bind and sequester p27. An increase in Cdk2 activity during G1 causes the degradation of p27. Phosphorylation of p27 is necessary for its degradation and is mediated by the SCF complex [Skp1,2/Cdc53 (cullins)]/F-box proteins. SCF complexes are involved in targeting phosphorylated proteins for ubiquitin-dependent proteolysis. The antibodies recognize mouse p27. A peptide coupled to KLH and corresponding to amino acids 184-197 at the C-terminus of mouse p27 was used as immunogen.
Synonyms: p27 (Kip1)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 27 kDa

Comments:

Related Products: ABIN968538, ABIN967635

Application Details

Purity: Serum

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Western blot analysis of p27. Lysates from NIH/3T3 cells were probed with polyclonal rabbit anti-mouse p27 antibody (ABIN967635) at a dilution of 1:1000. p27 is identified as a protein of ~27 kDa.

anti-Cyclin-Dependent Kinase Inhibitor 1B (CDKN1B) (AA 184-197) antibody (Image 2)

Publications

Product: Lee, Reynisdóttir, Massagué: "Cloning of p57KIP2, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution." in: Genes & development, Vol. 9, Issue 6, pp. 639-49, 1995 (PubMed).

Vlach, Hennecke, Amati: "Phosphorylation-dependent degradation of the cyclin-dependent kinase inhibitor p27." in: The EMBO journal, Vol. 16, Issue 17, pp. 5334-44, 1997 (PubMed).

Johnson, Walker: "Cyclins and cell cycle checkpoints." in: Annual review of pharmacology and toxicology, Vol. 39, pp. 295-312, 1999 (PubMed).

Tsvetkov, Yeh, Lee et al.: "p27(Kip1) ubiquitination and degradation is regulated by the SCF(Skp2) complex through phosphorylated Thr187 in p27." in: Current biology : CB, Vol. 9, Issue 12, pp. 661-4, 1999 (PubMed).