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CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (AA 184-197)

Details for Product anti-CDKN1B Antibody No. ABIN967635, Supplier: Login to see
Antigen
  • CDKN1B
  • CIB
  • CIBP
  • KIP1
  • PRKDCIP
  • SIP2-28
  • p27Kip1
  • AA408329
  • AI843786
  • Kip1
  • p27
  • CDKN4
  • Cdki1b
  • P27KIP1
  • MEN1B
  • MEN4
  • cdkn1bl
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • FAM14D
  • ISG12
  • ISG12A
  • P27
  • 1500011J20Rik
  • 2610202L11Rik
  • Bridge-1
Alternatives
anti-Mouse (Murine) CDKN1B antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 184-197
111
60
44
36
28
23
21
20
20
20
20
16
15
15
14
14
13
13
9
8
8
8
6
6
6
6
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
591
202
145
21
21
16
10
7
6
5
3
2
2
2
2
1
1
Host
Rabbit
504
99
37
4
2
1
Clonality
Polyclonal
Conjugate
This CDKN1B antibody is un-conjugated
31
29
27
25
25
25
6
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
439
305
184
103
96
80
47
30
25
16
14
7
3
1
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Mouse p27 aa. 184-197 peptide
Isotype Ig Fraction
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Serum
Alternative Name p27 Kip1 (CDKN1B Antibody Abstract)
Background P27 (Kip1) is a cyclin-dependent kinase inhibitor (cdkI) that associates with cyclin/cdk complexes to inhibit their catalytic activity. p27 was first identified as a result of its role in TGF-beta-induced G1 phase arrest and cell-cell contact. In vitro, p27 binds tightly to cyclin D-Cdk4, cyclin E-Cdk2, and cyclin A-Cdk2 complexes to inhibit their activity. In non-dividing cells, the level of p27 is elevated and its activity gradually decreases as cells reach S phase. Mitogenic stimulation causes the cyclin D-CDK complex to bind and sequester p27. An increase in Cdk2 activity during G1 causes the degradation of p27. Phosphorylation of p27 is necessary for its degradation and is mediated by the SCF complex [Skp1,2/Cdc53 (cullins)]/F-box proteins. SCF complexes are involved in targeting phosphorylated proteins for ubiquitin-dependent proteolysis. The antibodies recognize mouse p27. A peptide coupled to KLH and corresponding to amino acids 184-197 at the C-terminus of mouse p27 was used as immunogen.
Synonyms: p27 (Kip1)
Molecular Weight 27 kDa
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Comment

Related Products: ABIN968538, ABIN967635

Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (AA 184-197) (ABIN967635) Western blot analysis of p27. Lysates from NIH/3T3 cells were probed with polyclonal ...
 image for anti-CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (AA 184-197) (ABIN967635) anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 184-197) antibody (Image 2)
Product cited in: Tsvetkov, Yeh, Lee et al.: "p27(Kip1) ubiquitination and degradation is regulated by the SCF(Skp2) complex through phosphorylated Thr187 in p27." in: Current biology : CB, Vol. 9, Issue 12, pp. 661-4, 1999 (PubMed).

Johnson, Walker: "Cyclins and cell cycle checkpoints." in: Annual review of pharmacology and toxicology, Vol. 39, pp. 295-312, 1999 (PubMed).

Vlach, Hennecke, Amati: "Phosphorylation-dependent degradation of the cyclin-dependent kinase inhibitor p27." in: The EMBO journal, Vol. 16, Issue 17, pp. 5334-44, 1997 (PubMed).

Lee, Reynisdóttir, Massagué: "Cloning of p57KIP2, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution." in: Genes & development, Vol. 9, Issue 6, pp. 639-49, 1995 (PubMed).