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MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (C-Term)

Details for Product anti-MCL1 Antibody No. ABIN967637, Supplier: Log in to see
Antigen
  • AW556805
  • bcl2-L-3
  • BCL2L3
  • EAT
  • mcl-1
  • Mcl-1
  • MCL1-ES
  • mcl1/EAT
  • MCL1L
  • MCL1S
  • TM
Alternatives
anti-Human MCL-1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
C-Term
31
23
22
15
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13
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13
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9
5
3
3
3
3
2
2
2
2
1
1
1
1
1
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1
1
1
1
1
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1
Reactivity
Human
290
102
68
9
8
5
4
3
2
2
1
1
Host
Mouse
205
75
18
2
Clonality (Clone)
Monoclonal ()
Conjugate
This MCL-1 antibody is un-conjugated
11
9
9
6
6
5
3
2
2
2
2
2
2
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
244
118
102
58
55
31
26
22
20
8
7
6
1
Supplier
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Brand BD Pharmingen™
Immunogen Purified recombinant human Mcl-1 lacking the C-terminal hydrophobic domain
Clone 22-Mcl
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Mcl-1 (MCL1 Antibody Abstract)
Background The Bcl-2 family of proteins plays a pivotal role in determining the life and death of a cell. Members of the Bcl-2 family all possess at least one of four conserved Bcl-2 homology domains (BH1-BH4) which mediate protein-protein interactions. They can be divided into molecules that have either an anti-apoptotic or pro-apoptotic function. The ratio between these two groups of molecules can help determine whether a cell lives or dies. The majority of anti-apoptotic family members contain common BH1-BH4 domains and are most similar to Bcl-2. Mcl-1 is a member of the anti-apoptotic Bcl-2 group of proteins, including Bcl-x, Bcl-w, A1/Bfl-1, Boo/Diva, and Nr-13. Mcl-1 was cloned from the ML-1 human myeoblastic leukemia cell line. Mcl-1 localizes to the mitochrondria, like Bcl-2, but also associates with membranes via its C-terminal hydrophobic domain. Further studies have shown that Mcl-1 is upregulated in response to the GM-CSF and IL-3 cytokine signaling pathways and that this regulation of Mcl-1 is controlled at the level of transcription. Mcl-1 is detected at approximately 40 kDa in SDS/PAGE.
Molecular Weight 40 kDa
Research Area Cancer, Apoptosis/Necrosis
Pathways MAPK Signaling
Application Notes The antibody is tested by Western blot on K-562 cell lysate (ATCC CCL-243). Other applications include immunoprecipitation (1-2 µg/ml).
Comment

Related Products: ABIN968586, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Supplier Images
Western Blotting (WB) image for anti-MCL-1 antibody (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1) (C-Term) (ABIN967637) Western blot analysis of Mcl-1. Lysates from K-562 cells were probed with anti-human ...
Product cited in: Gross, McDonnell, Korsmeyer: "BCL-2 family members and the mitochondria in apoptosis." in: Genes & development, Vol. 13, Issue 15, pp. 1899-911, 1999

Wang, Chao, Chen, Kuo, Yen, Yang-Yen: "The antiapoptotic gene mcl-1 is up-regulated by the phosphatidylinositol 3-kinase/Akt signaling pathway through a transcription factor complex containing CREB." in: Molecular and cellular biology, Vol. 19, Issue 9, pp. 6195-206, 1999

Adams, Cory: "The Bcl-2 protein family: arbiters of cell survival." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1322-6, 1998

Yang, Kozopas, Craig: "The intracellular distribution and pattern of expression of Mcl-1 overlap with, but are not identical to, those of Bcl-2." in: The Journal of cell biology, Vol. 128, Issue 6, pp. 1173-84, 1995

Kozopas, Yang, Buchan, Zhou, Craig: "MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3516-20, 1993

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993