FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific))

Details for Product anti-FUT4 Antibody No. ABIN967638, Supplier: Log in to see
Antigen
  • AI451562
  • CD15
  • FAL
  • FucT-IV
  • LeX
  • SSEA-1
  • Ssea1
  • ELFT
  • FCT3A
  • FUC-TIV
  • FUTIV
  • Fuct
  • fucosyltransferase 4
  • fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
  • Fut4
  • FUT4
Reactivity
Human
525
137
91
15
15
3
1
Host
Mouse
445
100
Clonality (Clone)
Monoclonal ()
Conjugate
This FUT4 antibody is un-conjugated
63
38
32
14
14
14
14
11
11
10
9
7
7
7
7
6
6
6
5
5
5
5
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), ELISA
424
150
118
103
74
69
59
55
37
13
13
11
9
7
7
6
4
3
2
2
1
1
1
1
Options
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Brand BD Pharmingen™
Immunogen U-937 histiocytic cell line
Clone MMA
Isotype IgM, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD15 (FUT4 Antibody Abstract)
Background Reacts with the Le[x] blood group antigen, lacto-N-fucopentaose III or the CD15 antigen. The Le[x] determinant is expressed on normal cells and tissues of diverse origin, including myelomonocytic cells, myoepithelial cells in prostate, glandular epithelial cells including breast, renal cell and hepatocellular carcinomas, lung and urinary bladder adenocarcinomas and Reed-Sternberg cells in Hodgkin's disease.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Functional Studies:
Anti-Lewis[x] monoclonal antibody (clone MMA) has been used to determine Le[x] expression on normal and neoplastic cells in studies of tumor metastasis, together with sialyl-Le[x] (CD15s), E-selectin (CD62E) and Pselectin (CD62P) monoclonal antibodies.

Immunohistology:
Anti-Lewis[x] monoclonal antibody (clone MMA) can be used in staining formalin-fixed paraffin-embedded tissue sections of normal prostate, colon, stomach, brain and lymphoid tissues, adenocarcinomas of lung and urinary bladder. The MMA monoclonal antibody also stains neuraminidase-treated, formalin-fixed, paraffin-embedded sections of normal, metaplastic and neoplastic cervical epithelium.

Immunoblotting:
Anti-Lewis[x] monoclonal antibody (clone MMA) can be used to detect Le[x] in membranous glycoprotein cell or tissue extracts by immunoblotting.

Cell ELISA:
Anti-Lewis[x] monoclonal antibody (clone MMA) can be used to detect Le[x] on HL-60 promyelocytic, HT-29 colon cancer or F9 teratocarcinoma cells.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Product cited in: Bevilacqua, Nelson: "Endothelial-leukocyte adhesion molecules in inflammation and metastasis." in: Thrombosis and haemostasis, Vol. 70, Issue 1, pp. 152-4, 1993 (PubMed).

García Prats, López Carreira, Martínez-González, Ballestín, Gil, De Prada: "Granular cell basal cell carcinoma. Light microscopy, immunohistochemical and ultrastructural study." in: Virchows Archiv. A, Pathological anatomy and histopathology, Vol. 422, Issue 2, pp. 173-7, 1993 (PubMed).

Groves, Allen, Ross, Ahsan, Barker, MacDonald: "Expression of selectin ligands by cutaneous squamous cell carcinoma." in: The American journal of pathology, Vol. 143, Issue 4, pp. 1220-5, 1993 (PubMed).

Ma, Zarbo, Frierson, Lee: "Comparative immunohistochemical study of primary and metastatic carcinomas of the liver." in: American journal of clinical pathology, Vol. 99, Issue 5, pp. 551-7, 1993 (PubMed).

Narita, Funahashi, Satoh, Watanabe, Sakamoto, Takagi: "Association of expression of blood group-related carbohydrate antigens with prognosis in breast cancer." in: Cancer, Vol. 71, Issue 10, pp. 3044-53, 1993 (PubMed).

Paties, Taccagni, Papotti, Valente, Zangrandi, Aloi: "Apocrine carcinoma of the skin. A clinicopathologic, immunocytochemical, and ultrastructural study." in: Cancer, Vol. 71, Issue 2, pp. 375-81, 1993 (PubMed).

Torii, Nakayama, Harada, Nakao, Nonami, Sakamoto, Watanabe, Ito, Takagi: "Expression of the CD15 antigen in hepatocellular carcinoma." in: Cancer, Vol. 71, Issue 12, pp. 3864-7, 1993 (PubMed).

McCormick, Zetter: "Adhesive interactions in angiogenesis and metastasis." in: Pharmacology & therapeutics, Vol. 53, Issue 2, pp. 239-60, 1992 (PubMed).

Grignon, Ro, Ayala, Johnson, Ordóñez: "Primary adenocarcinoma of the urinary bladder. A clinicopathologic analysis of 72 cases." in: Cancer, Vol. 67, Issue 8, pp. 2165-72, 1991 (PubMed).

Zhou, Moore, Smith, Varki, McEver, Cummings: "The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells." in: The Journal of cell biology, Vol. 115, Issue 2, pp. 557-64, 1991 (PubMed).

OHara, Corson, Pinkus, Stahel: "ME1. A monoclonal antibody that distinguishes epithelial-type malignant mesothelioma from pulmonary adenocarcinoma and extrapulmonary malignancies." in: The American journal of pathology, Vol. 136, Issue 2, pp. 421-8, 1990 (PubMed).

Hsu, Huang, Hsu, Ge, Ho, Cuttita, Mulshine: "Biochemical and ultrastructural study of Leu M1 antigen in Reed-Sternberg cells: comparison with granulocytes and interdigitating reticulum cells." in: Journal of the National Cancer Institute, Vol. 77, Issue 2, pp. 363-70, 1986 (PubMed).

Hanjan, Kearney, Cooper: "A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells." in: Clinical immunology and immunopathology, Vol. 23, Issue 2, pp. 172-88, 1982 (PubMed).