CD53 (CD53) antibody

Details for Product No. ABIN967642
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Synonyms AI323659, Ox-44, Tspan25, MOX44, TSPAN25, OX44
Mouse (Murine)
(95), (25), (17)
(93), (14), (6)
Clonality (Clone)
Monoclonal ()
(21), (13), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
(96), (45), (39), (27), (15), (15), (10), (8), (7), (4), (3), (2), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 0.5 mg
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Catalog No. ABIN967642
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Immunogen BAB/14 mouse macrophage cell line RAW 264
Clone OX-79
Isotype IgM, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD53
Background The OX-79 antibody reacts with CD53, a 35-45-kDa member of the Transmembrane 4-pass protein superfamily (TM4SF). As in the human, mouse peripheral leukocytes express CD53 mRNA and protein, and erythrocytes and nonhematopoietic cells do not. However, the distribution of CD53 in the mouse and rat thymus differs from that in the human. In the mouse, most CD4+8- and major subsets of CD4- CD8- (double-negative) and CD4- CD8+ thymocytes express CD53, while most cortical CD4+ CD8+ (double-positive) thymocytes are CD53-low or CD53-negative. CD53 expression can be induced in double-positive thymocytes by cross-linking of their T-cell receptors with anti-TCR mAb or peptide-pulsed antigen-presenting cells. There is a strong correlation between positive selection of thymocytes and CD53 expression. Its association with CD2 and a tyrosine phosphatase in rat T lymphocytes and with CD19, CD21, HLA-DR, and other TM4SF proteins in human B lymphocytes suggests that CD53 is involved in leukocyte signal transduction.
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Other reported applications include western blot analysis and immunohistochemical (IHC) staining of acetone-fixed frozen sections. This antibody has been tested in development for IHC application on acetone-fixed frozen Balb/C mouse spleen and thymus tissue sections with the antobody concentration of 1-10 µg/ml. IHC of paraffin-embedded sections is not recommended.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Puls, Hogquist, Reilly et al.: "CD53, a thymocyte selection marker whose induction requires a lower affinity TCR-MHC interaction than CD69, but is up-regulated with slower kinetics." in: International immunology, Vol. 14, Issue 3, pp. 249-58, 2002 (PubMed).

Angelisová, Hilgert, Horejsí: "Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins." in: Immunogenetics, Vol. 39, Issue 4, pp. 249-56, 1994 (PubMed).

Carmo, Wright: "Association of the transmembrane 4 superfamily molecule CD53 with a tyrosine phosphatase activity." in: European journal of immunology, Vol. 25, Issue 7, pp. 2090-5, 1995 (PubMed).

Tomlinson, Hanke, Hughes et al.: "Characterization of mouse CD53: epitope mapping, cellular distribution and induction by T cell receptor engagement during repertoire selection." in: European journal of immunology, Vol. 25, Issue 8, pp. 2201-5, 1995 (PubMed).

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