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MCM4 antibody (Minichromosome Maintenance Deficient 4) (AA 21-40)

Details for Product anti-MCM4 Antibody No. ABIN967643, Supplier: Log in to see
Antigen
  • cb1025
  • fc12c09
  • fj85g09
  • hm:zeh1616
  • wu:fc12c09
  • wu:fj85g09
  • zeh1616
  • CDC21
  • CDC54
  • NKCD
  • NKGCD
  • P1-CDC21
  • hCdc21
  • Mcmd4
  • 19G
  • AI325074
  • AU045576
  • Cdc21
  • mKIAA4003
  • mcdc21
  • minichromosome maintenance complex component 4
  • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae)
  • minichromosome maintenance deficient 4 homolog (S. cerevisiae)
  • mcm4
  • MCM4
  • Mcm4
Alternatives
anti-Human MCM4 antibody for Western Blotting
Epitope
AA 21-40
19
14
9
8
6
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
114
58
40
10
10
8
7
6
5
4
4
4
3
2
2
2
Host
Rabbit
98
8
8
Clonality
Polyclonal
Conjugate
This MCM4 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
92
38
37
19
13
13
3
3
2
1
Options
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Immunogen Human MCM4 Peptide
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Serum
Alternative Name MCM4 (MCM4 Antibody Abstract)
Background Minichromosome maintenance (MCM) proteins play an essential role for the initiation of eukaryotic DNA replication and bind with chromatin in vivo. They act as factors that ‘license’ DNA for exactly one round of replication per cell cycle. MCM proteins share a highly conserved domain of about 210 amino-acid residues. This domain shares some similarities with that of various other families of DNA-dependent ATPases which implies that MCM proteins may be involved in an ATP-consuming step in the initiation of DNA replication. Eukaryotes possess a family of six MCM proteins (MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7) that contain this domain. MCM proteins were first identified in yeast where most of them have a direct role in the initiation of chromosomal DNA replication by interacting directly with autonomously replicating sequences (ARS). They were thus called 'minichromosome maintenance proteins' with gene symbols pre-fixed by MCM. Human MCM4, also called CDC21 or CDC54, is a nuclear protein that is highly phosphorylated in mitotic cells. The phosphorylated form of MCM4 appears to be less tightly bound to nuclear structures than the underphosphorylated form suggesting that phosphorylation/ dephosphorylation reactions may determine the nuclear distribution of the protein. In HeLa cells, MCM protein levels remain relatively constant except for fluctuations during the G0 to G1/S period whereby in G0, MCM2 and MCM5 are found at much lower concentrations when compared to MCM3 and MCM7.
Synonyms: Minichromosome Maintenance Protein 4
Molecular Weight 97 kDa
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Pathways DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding
Comment

Related Products: ABIN967390

Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-MCM4 antibody (Minichromosome Maintenance Deficient 4) (AA 21-40) (ABIN967643) Western blot analysis of human minichromosome maintenance proteins (MCM). HeLa cell (...
 image for anti-MCM4 antibody (Minichromosome Maintenance Deficient 4) (AA 21-40) (ABIN967643) anti-Minichromosome Maintenance Deficient 4 (MCM4) (AA 21-40) antibody (Image 2)
Product cited in: Tsuruga, Yabuta, Hashizume, Ikeda, Endo, Nojima: "Expression, nuclear localization and interactions of human MCM/P1 proteins." in: Biochemical and biophysical research communications, Vol. 236, Issue 1, pp. 118-25, 1997 (PubMed).

Musahl, Schulte, Burkhart, Knippers: "A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins." in: European journal of biochemistry / FEBS, Vol. 230, Issue 3, pp. 1096-101, 1995 (PubMed).

Hu, Burkhart, Schulte, Musahl, Knippers: "The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins." in: Nucleic acids research, Vol. 21, Issue 23, pp. 5289-93, 1994 (PubMed).

Coxon, Maundrell, Kearsey: "Fission yeast cdc21+ belongs to a family of proteins involved in an early step of chromosome replication." in: Nucleic acids research, Vol. 20, Issue 21, pp. 5571-7, 1993 (PubMed).

Koonin: "A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication." in: Nucleic acids research, Vol. 21, Issue 11, pp. 2541-7, 1993 (PubMed).