Minichromosome Maintenance Deficient 4 (MCM4) (AA 21-40) antibody

Antigen: Minichromosome Maintenance Deficient 4 (MCM4)

Synonyms: CDC21, CDC54, hCdc21, MGC33310, P1-CDC21, mcm4-A, cdc21, MGC98251, mcm4, MGC69358, MCM4, MGC138087, MGC140484

Binding Site: AA 21-40

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967643

Additional Information

Alternative name: MCM4

Immunogen: Human MCM4 Peptide

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Description: Minichromosome maintenance (MCM) proteins play an essential role for the initiation of eukaryotic DNA replication and bind with chromatin in vivo. They act as factors that ‘license’ DNA for exactly one round of replication per cell cycle. MCM proteins share a highly conserved domain of about 210 amino-acid residues. This domain shares some similarities with that of various other families of DNA-dependent ATPases which implies that MCM proteins may be involved in an ATP-consuming step in the initiation of DNA replication. Eukaryotes possess a family of six MCM proteins (MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7) that contain this domain. MCM proteins were first identified in yeast where most of them have a direct role in the initiation of chromosomal DNA replication by interacting directly with autonomously replicating sequences (ARS). They were thus called 'minichromosome maintenance proteins' with gene symbols pre-fixed by MCM. Human MCM4, also called CDC21 or CDC54, is a nuclear protein that is highly phosphorylated in mitotic cells. The phosphorylated form of MCM4 appears to be less tightly bound to nuclear structures than the underphosphorylated form suggesting that phosphorylation/ dephosphorylation reactions may determine the nuclear distribution of the protein. In HeLa cells, MCM protein levels remain relatively constant except for fluctuations during the G0 to G1/S period whereby in G0, MCM2 and MCM5 are found at much lower concentrations when compared to MCM3 and MCM7.
Synonyms: Minichromosome Maintenance Protein 4

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Molecular Weight: 97 kDa

Comments:

Related Products: ABIN967390

Application Details

Purity: Serum

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis of human minichromosome maintenance proteins (MCM). HeLa cell (Human cervical epitheloid carcinoma, ATCC CCL-2) nuclear extracts were probed with antibodies to MCM2 (lane 1), MCM3 (lane 2), MCM4 (lane 3), MCM5 (lane 4), MCM6 (lane 5), or MCM7 (lane 6) using dilutions of 1:2000-1:6000.

anti-Minichromosome Maintenance Deficient 4 (MCM4) (AA 21-40) antibody (Image 2)

Publications

Product: Coxon, Maundrell, Kearsey: "Fission yeast cdc21+ belongs to a family of proteins involved in an early step of chromosome replication." in: Nucleic acids research, Vol. 20, Issue 21, pp. 5571-7, 1993 (PubMed).

Musahl, Schulte, Burkhart et al.: "A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins." in: European journal of biochemistry / FEBS, Vol. 230, Issue 3, pp. 1096-101, 1995 (PubMed).

Hu, Burkhart, Schulte et al.: "The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins." in: Nucleic acids research, Vol. 21, Issue 23, pp. 5289-93, 1994 (PubMed).

Koonin: "A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication." in: Nucleic acids research, Vol. 21, Issue 11, pp. 2541-7, 1993 (PubMed).

Tsuruga, Yabuta, Hashizume et al.: "Expression, nuclear localization and interactions of human MCM/P1 proteins." in: Biochemical and biophysical research communications, Vol. 236, Issue 1, pp. 118-25, 1997 (PubMed).