Phosphatase and Tensin Homolog (PTEN) (AA 387-400) antibody

Antigen: Phosphatase and Tensin Homolog (PTEN)

Synonyms: BZS, MHAM, TEP1, MMAC1, PTEN1, 10q23del, MGC11227, AI463227, MGC183880, 2310035O07Rik, A130070J02Rik, B430203M17Rik, Mmac, PTEN, bzs, mham, tep1, mmac1, pten1, Pten

Binding Site: AA 387-400

Clonality: Monoclonal (A2B1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967644

Additional Information

Alternative name: PTEN

Immunogen: Human PTEN peptide

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: A2B1

Description: Cancer can develop when cells escape normal growth control mechanisms through mutations in proto-oncogenes or tumor suppressor genes. A characteristic of most oncogene and tumor suppressor gene products is that they are components of signal transduction pathways that are essential for maintaining cellular homeostasis. PTEN [phosphatase and tensin homolog detected on chromosome ten/MMAC or TEP1 (mutated in multiple advanced cancers] is a tumor suppressor gene that has been identified as mutated in multiple tumor types. PTEN is a phosphatase that acts on proteins and lipid second messengers. The ability of PTEN to cause tumors is thought to result from the loss of its negative control on the phosphoinositide 3-kinase signaling pathway which regulates cell growth and survival. Using a mutant mouse model for PTEN, Suzuki et al. demonstrated that these mice had elevated levels of the protein kinase Akt in tumors associated with loss of heterozygosity of mPTEN. Human PTEN is a polypeptide of 403 amino acids based on the nucleotide sequence and is observed to migrate at ~55 kDa in SDS/PAGE. The antibody recognizes human and mouse PTEN. A peptide representing amino acids 387-400 at the C-terminus of human PTEN was used as the immunogen.
Synonyms: MMAC1, TEP-1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 55 kDa

Comments:

Related Products: ABIN967389

Application Details

Application Notes: WI-38 cells are recommended as a positive control.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4° C.

Research Area: Cancer, Cell Cycle, Transcription Factors, Kinases/Phosphatases, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of PTEN. Lysates from the WI-38 cell line were probed with purified anti-PTEN antibody at a concentration of 6.0 (lane 1), 2.0 (lane 2), or 0.5 ìg/ml (lane 3). PTEN is identified as a protein of ~55 kDa.

anti-Phosphatase and Tensin Homolog (PTEN) (AA 387-400) antibody (Image 2)

Publications

Product: Steck, Pershouse, Jasser et al.: "Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers." in: Nature genetics, Vol. 15, Issue 4, pp. 356-62, 1997 (PubMed).

Suzuki, de la Pompa, Stambolic et al.: "High cancer susceptibility and embryonic lethality associated with mutation of the PTEN tumor suppressor gene in mice." in: Current biology : CB, Vol. 8, Issue 21, pp. 1169-78, 1998 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).