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APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 12-28)

Details for Product anti-APAF1 Antibody No. ABIN967648, Supplier: Log in to see
Antigen
  • 6230400I06Rik
  • anon-53Fa
  • apaf-1
  • Apaf-1
  • APAF-1
  • Apaf1
  • apaf1
  • APAF1
  • Apaf1l
  • arc
  • ARK
  • ark
  • CED4
  • CG6829
  • D-Apaf-1
  • dapaf
  • Dapaf-1
  • dAPAF-1
  • dapaf-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • Dapaf-1/HAC-1
  • dapaf-1L
  • dapaf-1S
  • dApaf1
  • DARK
  • dark
  • dArk
  • Dark
  • Dark/Apaf-I
  • dark/dapaf-1/hac-1
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • dark/hac-1/dapaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • fog
  • hac-1
  • Hac-1
  • Hac-1/Dark
  • hac1
  • Hac1
  • l(2)SH0173
  • mKIAA0413
  • T1
  • zgc:136543
Alternatives
anti-Human APAF1 antibody for Western Blotting
Epitope
AA 12-28
46
44
16
13
13
8
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
200
109
91
4
2
2
2
1
1
1
Host
Rabbit
172
25
13
3
Clonality
Polyclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Western Blotting (WB)
157
71
64
52
34
22
21
13
9
5
4
Supplier
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Brand BD Pharmingen™
Immunogen Human APAF-1 aa. 12-28 Peptide
Isotype Ig Fraction
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification The polyclonal antibody was purified from antiserum by affinity chromatography.
Alternative Name Apaf-1 (APAF1 Antibody Abstract)
Background Apoptosis can be induced by a family of cell death receptors (tumor necrosis factor) and their respective ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. Another mechanism of inducing apoptosis involves the participation of the mitochrondria in which various forms of cellular stress can trigger the release of cytochrome c from the intermembrane space (IMS) of the mitochrondria into the cytoplasm. The mammalian homologue of the CED-4 cell death gene in C. elegans has been identified from human and mouse and designated APAF-1 (for Apoptosis Protease Activating Factor-1). Cytochrome c associates with APAF-1 in the presence of dATP or ATP and induces an oligomerization of APAF-1 which then binds procaspase-9, forming an apoptosome” containing APAF-1, cytochrome c and procaspase-9. Recent studies have shown that dATP/ATP hydrolysis is necessary for APAF-1 to interact with cytochrome c and bind to procaspase-9. Procaspase-9 is then processed via an intrinsic autocatalytic activity of itself, triggered by APAF-1, which now allows caspase-9 to activate the downstream executioners caspase-3, -6 and -7. This antibody has been reported to recognize both human and mouse APAF-1. A peptide corresponding to amino acids 12 to 28 of human APAF-1 was used as the immunogen where the sequence of this peptide have been reported to be identical between both human and mouse.
Synonyms: Apoptosis Protease Activating Factor-1
Molecular Weight 130 kDa
Research Area Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis
Comment

Related Products: ABIN968535, ABIN967390

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Supplier Images
Western Blotting (WB) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 12-28) (ABIN967648) Western blot analysis of APAF-1. HeLa cell lysates (Human cervical epitheloid carcino...
 image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 12-28) (ABIN967648) anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 12-28) antibody (Image 2)
Western Blotting (WB) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 12-28) (ABIN967648) anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 12-28) antibody (Image 3)
Product cited in: Hu, Benedict, Ding, Núñez: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).

Cecconi, Alvarez-Bolado, Meyer, Roth, Gruss: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998 (PubMed).

Srinivasula, Ahmad, Fernandes-Alnemri, Alnemri: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998 (PubMed).

Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Zou, Henzel, Liu, Lutschg, Wang: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).