Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 12-28) antibody
Alternatives Western Blotting (WB)
|6 references available|
|Quantity||50 µg (0.5 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Human APAF-1 Peptide|
Apoptosis can be induced by a family of cell death receptors (tumor necrosis factor) and their respective ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. Another mechanism of inducing apoptosis involves the participation of the mitochrondria in which various forms of cellular stress can trigger the release of cytochrome c from the intermembrane space (IMS) of the mitochrondria into the cytoplasm. The mammalian homologue of the CED-4 cell death gene in C. elegans has been identified from human and mouse and designated APAF-1 (for Apoptosis Protease Activating Factor-1). Cytochrome c associates with APAF-1 in the presence of dATP or ATP and induces an oligomerization of APAF-1 which then binds procaspase-9, forming an apoptosome” containing APAF-1, cytochrome c and procaspase-9. Recent studies have shown that dATP/ATP hydrolysis is necessary for APAF-1 to interact with cytochrome c and bind to procaspase-9. Procaspase-9 is then processed via an intrinsic autocatalytic activity of itself, triggered by APAF-1, which now allows caspase-9 to activate the downstream executioners caspase-3, -6 and -7. This antibody has been reported to recognize both human and mouse APAF-1. A peptide corresponding to amino acids 12 to 28 of human APAF-1 was used as the immunogen where the sequence of this peptide have been reported to be identical between both human and mouse.
Synonyms: Apoptosis Protease Activating Factor-1
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
|Molecular Weight||130 kDa|
Related Products: ABIN968535, ABIN967390
|Synonyms||CED4, APAF-1, DKFZp781B1145, fog, Apaf-1, Apaf1l, KIAA0413, mKIAA0413, 6230400I06Rik, ARK, Apaf1, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Hac-1, Hac-1/Dark, Hac1, T1, anon-53Fa, arc, dApaf-1/DARK/HAC-1, dApaf1, dapaf, dapaf-1L, dapaf-1S, dark/dapaf-1/hac-1, DmelCG6829, CG6829, apaf-1, MGC80868, APAF1|
|Purification||Purified from antiserum by affinity chromatography.|
|Buffer||Aqueous buffered solution.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at 4° C.|
|Restrictions||For Research Use only|
Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).
Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).
Srinivasula, Ahmad, Fernandes-Alnemri et al.: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998 (PubMed).
Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998 (PubMed).
Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).
Hu, Benedict, Ding et al.: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).
|Hosts||Rabbit (61), Mouse (11), Rat (4), Chicken (1)|
|Reactivities||Human (70), Mouse (Murine) (33), Rat (Rattus) (26), Cow (Bovine) (2), Gerbil (1), Guinea Pig (1), Hamster (1), Horse (Equine) (1), Monkey (1), Rabbit (1)|
|Applications||Western Blotting (WB) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (23), Immunohistochemistry (IHC) (22), ELISA (21), Immunoprecipitation (IP) (14), Immunocytochemistry (ICC) (6), Immunofluorescence (IF) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunohistochemistry (Fixed) (IHC (fx)) (4), Fluorescence Microscopy (FM) (1)|
|Epitopes||C-Term (15), N-Term (8), AA 12-28 (6), AA 1-97 (2), AA 1-464 (1), AA 264-282 (1), AA 307-388 (1), AA 451-468 (1)|