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Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 12-28) antibody

Details for Product No. ABIN967648
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Antigen
Synonyms
APAF-1, CED4, 6230400I06Rik, Apaf-1, Apaf1l, fog, mKIAA0413, apaf-1, zgc:136543, APAF1, ARK, Apaf1, CG6829, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dAp ... show more
APAF-1, CED4, 6230400I06Rik, Apaf-1, Apaf1l, fog, mKIAA0413, apaf-1, zgc:136543, APAF1, ARK, Apaf1, CG6829, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Dark/Hac-1/dApaf1, Dmel\\CG6829, Hac-1, Hac-1/Dark, Hac1, T1, anon-53Fa, apaf1, arc, ark, dAPAF-1, dApaf-1, dApaf-1/DARK/HAC-1, dApaf1, dArk, dapaf, dapaf-1, dapaf-1L, dapaf-1S, dark, dark/dapaf-1/hac-1, dark/hac-1/dapaf-1, hac-1, hac1, l(2)SH0173 show less
Epitope
AA 12-28
(46), (35), (9), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(162), (90), (80), (24), (15), (12), (1), (1), (1), (1), (1)
Host
Rabbit
(131), (36), (6), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB)
(121), (54), (52), (42), (20), (20), (11), (10), (7), (3), (1), (1)
Pubmed 6 references available
Quantity 50 µg
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Catalog No. ABIN967648
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Immunogen Human APAF-1 Peptide
Isotype Ig
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from antiserum by affinity chromatography.
Purity Purified
Alternative Name Apaf-1
Background Apoptosis can be induced by a family of cell death receptors (tumor necrosis factor) and their respective ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. Another mechanism of inducing apoptosis involves the participation of the mitochrondria in which various forms of cellular stress can trigger the release of cytochrome c from the intermembrane space (IMS) of the mitochrondria into the cytoplasm. The mammalian homologue of the CED-4 cell death gene in C. elegans has been identified from human and mouse and designated APAF-1 (for Apoptosis Protease Activating Factor-1). Cytochrome c associates with APAF-1 in the presence of dATP or ATP and induces an oligomerization of APAF-1 which then binds procaspase-9, forming an apoptosome” containing APAF-1, cytochrome c and procaspase-9. Recent studies have shown that dATP/ATP hydrolysis is necessary for APAF-1 to interact with cytochrome c and bind to procaspase-9. Procaspase-9 is then processed via an intrinsic autocatalytic activity of itself, triggered by APAF-1, which now allows caspase-9 to activate the downstream executioners caspase-3, -6 and -7. This antibody has been reported to recognize both human and mouse APAF-1. A peptide corresponding to amino acids 12 to 28 of human APAF-1 was used as the immunogen where the sequence of this peptide have been reported to be identical between both human and mouse.
Synonyms: Apoptosis Protease Activating Factor-1
Molecular Weight 130 kDa
Research Area Apoptosis/Necrosis
Comment

Related Products: ABIN968535, ABIN967390

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 12-28) antibody Western blot analysis of APAF-1. HeLa cell lysates (Human cervical epitheloid carcinoma, ATCC CCL-2.2) were probed with the polyclonal rabbit anti- APAF-1 antibody at a concentration of 0.5 µg/ml (lane 1), 0.25 µg/ml (lane 2) or in the presence of blocking peptide (lane 3). APAF-1 is identified as a band migrating at ~ 130 kD on HeLa cell lysates.
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 12-28) antibody (2) anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 12-28) antibody (Image 2)
Product cited in: Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Li, Nijhawan, Budihardjo et al.: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).

Srinivasula, Ahmad, Fernandes-Alnemri et al.: "Autoactivation of procaspase-9 by Apaf-1-mediated oligomerization." in: Molecular cell, Vol. 1, Issue 7, pp. 949-57, 1998 (PubMed).

Green, Reed: "Mitochondria and apoptosis." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1309-12, 1998 (PubMed).

Cecconi, Alvarez-Bolado, Meyer et al.: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Hu, Benedict, Ding et al.: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, 1999 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967648
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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