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Thioredoxin (TXN) antibody

Details for Product No. ABIN967658, Supplier: Log in to see
Antigen
  • trx
  • TXN
  • ARABIDOPSIS THALIANA THIOREDOXIN H-TYPE 1
  • ATTRX H1
  • ATTRX1
  • THIOREDOXIN
  • thioredoxin H-type 1
  • ATH5
  • ATTRX5
  • F27F5.21
  • F27F5_21
  • LIV1
  • LOCUS OF INSENSITIVITY TO VICTORIN 1
  • THIOREDOXIN H-TYPE 5
  • thioredoxin H-type 5
  • SCM1.18
  • trxA3
  • thioredoxin
  • TRDX
  • TRX
  • TRX1
  • ADF
  • AW550880
  • Trx1
  • Txn
  • hypothetical protein
  • thioredoxin
  • thioredoxin H1
  • thioredoxin H5
  • thioredoxin-like protein
  • acrocallosal syndrome
  • peptidase C
  • thioredoxin 1
  • thioredoxin
  • Thioredoxin
  • txn
  • TXN
  • TRX1
  • TRX5
  • trx
  • SCO0885
  • trx2
  • PHG25ORF099c
  • ACLS
  • PEPC
  • LOC100349046
  • Txn
  • Txn1
Alternatives
anti-Human Thioredoxin antibody for Enzyme Immunoassay
Reactivity
Human
198
46
31
3
2
1
1
1
1
1
Host
Mouse
128
83
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
17
15
11
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Intracellular Staining (ICS), Western Blotting (WB)
196
143
63
51
29
28
25
15
5
2
1
1
1
Options
Supplier
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Brand BD Pharmingen™
Immunogen Recombinant human Trx
Clone 2G11-TRX
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Thioredoxin (TXN Antibody Abstract)
Background The immunogen used to raise the 2G11 hybridoma was recombinant full length human thioredoxin (Trx) is an intracellular, multifunctional 12 kD (104 a.a.) protein with reduction/oxidation (redox) activity expressed by most cells of the human body. Trx is also secreted via the leaderless pathway (i.e. like IL-1beta) by cells such as monocytes and lymphocytes upon activation or in response to oxidative stress. A truncated, 10 kD form of Trx (Trx1-84) has also been reported. Secreted Trx (also known as adult T-cell leukemia derived factor (ADF)) exhibits several cytokine- and chemokine-like functions such as activating eosinophils and granulocytes and chemotaxis for neutrophils, monocytes and T cells. 2G11 reacts exclusively with Trx(1-104), but not with its truncated form.
Synonyms: TRX (1-104)
Research Area Coagulation, Enzymes, Metabolism
Pathways Carbohydrate Homeostasis
Application Notes The 2G11 antibody is suitable to detect Trx (1-104) in western blots. 2G11 is useful for Intracellular Staining and Flow cytometry using appropriate conjugated secondary antibody. A useful control for demonstrating specificity of staining with conjugated 2G11 is to pre-block the fixed/permeabilized cells with unlabeled 2G11 antibody (ABIN967658) prior to staining.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4° C.
Supplier Images
Western Blotting (WB) image for anti-Thioredoxin (TXN) antibody (ABIN967658) Detection of Trx (1-104) by Western Blotting: Recombinant huTrx (100, 50 25 or 12.5 n...
 image for anti-Thioredoxin (TXN) antibody (ABIN967658) Expression of Trx by human peripheral blood mononuclear cells (PBMC). Human PBMC were...
 image for anti-Thioredoxin (TXN) antibody (ABIN967658) Staining with the FITC-2G11 antibody
Product cited in: Bertini, Howard, Dong, Oppenheim, Bizzarri, Sergi, Caselli, Pagliei, Romines, Wilshire, Mengozzi, Nakamura, Yodoi, Pekkari, Gurunath, Holmgren, Herzenberg, Herzenberg, Ghezzi: "Thioredoxin, a redox enzyme released in infection and inflammation, is a unique chemoattractant for neutrophils, monocytes, and T cells." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1783-9, 1999 (PubMed).

Yoshida, Katoh, Tetsuka, Uno, Matsui, Okamoto: "Involvement of thioredoxin in rheumatoid arthritis: its costimulatory roles in the TNF-alpha-induced production of IL-6 and IL-8 from cultured synovial fibroblasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 351-8, 1999 (PubMed).

Sahaf, Söderberg, Spyrou, Barral, Pekkari, Holmgren, Rosén: "Thioredoxin expression and localization in human cell lines: detection of full-length and truncated species." in: Experimental cell research, Vol. 236, Issue 1, pp. 181-92, 1997 (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).