Thioredoxin (TXN) antibody

Details for Product No. ABIN967658
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Antigen
Synonyms
trx, TXN, ARABIDOPSIS THALIANA THIOREDOXIN H-TYPE 1, ATTRX H1, ATTRX1, THIOREDOXIN, thioredoxin H-type 1, ATH5, ATTRX5, F27F5.21, F27F5_21, LIV1, LOCUS OF INSENSITIVITY TO VICTORIN 1, THIOREDOXIN H-TY ... show more
trx, TXN, ARABIDOPSIS THALIANA THIOREDOXIN H-TYPE 1, ATTRX H1, ATTRX1, THIOREDOXIN, thioredoxin H-type 1, ATH5, ATTRX5, F27F5.21, F27F5_21, LIV1, LOCUS OF INSENSITIVITY TO VICTORIN 1, THIOREDOXIN H-TYPE 5, thioredoxin H-type 5, SCM1.18, trxA3, thioredoxin, TRDX, TRX, TRX1, ADF, AW550880, Trx1, Txn show less
Reactivity
Human
(138), (20), (18), (6), (4), (1), (1), (1)
Host
Mouse
(86), (65), (3), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(11), (10), (8), (3), (3), (3)
Application
Western Blotting (WB), Intracellular Flow Cytometry (ICFC)
(145), (108), (40), (18), (13), (13), (11), (9), (7), (7), (2), (2), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Immunogen Recombinant human Trx
Clone 2G11
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Thioredoxin (TXN Antibody Abstract)
Background The immunogen used to raise the 2G11 hybridoma was recombinant full length human thioredoxin (Trx) is an intracellular, multifunctional 12 kD (104 a.a.) protein with reduction/oxidation (redox) activity expressed by most cells of the human body. Trx is also secreted via the leaderless pathway (i.e. like IL-1beta) by cells such as monocytes and lymphocytes upon activation or in response to oxidative stress. A truncated, 10 kD form of Trx (Trx1-84) has also been reported. Secreted Trx (also known as adult T-cell leukemia derived factor (ADF)) exhibits several cytokine- and chemokine-like functions such as activating eosinophils and granulocytes and chemotaxis for neutrophils, monocytes and T cells. 2G11 reacts exclusively with Trx(1-104), but not with its truncated form.
Synonyms: TRX (1-104)
Research Area Coagulation, Enzymes, Metabolism
Application Notes The 2G11 antibody is suitable to detect Trx (1-104) in western blots. 2G11 is useful for Intracellular Staining and Flow cytometry using appropriate conjugated secondary antibody. A useful control for demonstrating specificity of staining with conjugated 2G11 is to pre-block the fixed/permeabilized cells with unlabeled 2G11 antibody (ABIN967658) prior to staining.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Thioredoxin (TXN) antibody Detection of Trx (1-104) by Western Blotting: Recombinant huTrx (100, 50 25 or 12.5 n...
anti-Thioredoxin (TXN) antibody (2) Expression of Trx by human peripheral blood mononuclear cells (PBMC). Human PBMC were...
anti-Thioredoxin (TXN) antibody (3) Staining with the FITC-2G11 antibody
General Yoshida, Katoh, Tetsuka et al.: "Involvement of thioredoxin in rheumatoid arthritis: its costimulatory roles in the TNF-alpha-induced production of IL-6 and IL-8 from cultured synovial fibroblasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 351-8, 1999 (PubMed).

Bertini, Howard, Dong et al.: "Thioredoxin, a redox enzyme released in infection and inflammation, is a unique chemoattractant for neutrophils, monocytes, and T cells." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1783-9, 1999 (PubMed).

Sahaf, Söderberg, Spyrou et al.: "Thioredoxin expression and localization in human cell lines: detection of full-length and truncated species." in: Experimental cell research, Vol. 236, Issue 1, pp. 181-92, 1997 (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Validation Images
Catalog No. ABIN967658
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