Thioredoxin (TXN) antibody

Details for Product No. ABIN967658
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
trx, TXN, ARABIDOPSIS THALIANA THIOREDOXIN H-TYPE 1, ATTRX H1, ATTRX1, THIOREDOXIN, thioredoxin H-type 1, ATH5, ATTRX5, F27F5.21, F27F5_21, LIV1, LOCUS OF INSENSITIVITY TO VICTORIN 1, THIOREDOXIN H-TY ... show more
trx, TXN, ARABIDOPSIS THALIANA THIOREDOXIN H-TYPE 1, ATTRX H1, ATTRX1, THIOREDOXIN, thioredoxin H-type 1, ATH5, ATTRX5, F27F5.21, F27F5_21, LIV1, LOCUS OF INSENSITIVITY TO VICTORIN 1, THIOREDOXIN H-TYPE 5, thioredoxin H-type 5, SCM1.18, trxA3, thioredoxin, TRDX, TRX, TRX1, ADF, AW550880, Trx1, Txn show less
Reactivity
Human
(125), (27), (24), (12), (12), (12), (6), (4), (1), (1)
Host
Mouse
(81), (57), (4), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (8), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Intracellular Flow Cytometry (ICFC)
(122), (93), (21), (17), (15), (13), (12), (10), (9), (3)
Pubmed 4 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967658
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Recombinant human Trx
Clone 2G11
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Thioredoxin
Background The immunogen used to raise the 2G11 hybridoma was recombinant full length human thioredoxin (Trx) is an intracellular, multifunctional 12 kD (104 a.a.) protein with reduction/oxidation (redox) activity expressed by most cells of the human body. Trx is also secreted via the leaderless pathway (i.e. like IL-1beta) by cells such as monocytes and lymphocytes upon activation or in response to oxidative stress. A truncated, 10 kD form of Trx (Trx1-84) has also been reported. Secreted Trx (also known as adult T-cell leukemia derived factor (ADF)) exhibits several cytokine- and chemokine-like functions such as activating eosinophils and granulocytes and chemotaxis for neutrophils, monocytes and T cells. 2G11 reacts exclusively with Trx(1-104), but not with its truncated form.
Synonyms: TRX (1-104)
Research Area Coagulation, Enzymes, Metabolism
Application Notes The 2G11 antibody is suitable to detect Trx (1-104) in western blots. 2G11 is useful for Intracellular Staining and Flow cytometry using appropriate conjugated secondary antibody. A useful control for demonstrating specificity of staining with conjugated 2G11 is to pre-block the fixed/permeabilized cells with unlabeled 2G11 antibody (ABIN967658) prior to staining.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Thioredoxin (TXN) antibody Detection of Trx (1-104) by Western Blotting: Recombinant huTrx (100, 50 25 or 12.5 ng-lanes 1 -4, respectively) or dilutions of U937 cell lysate (5x10e4 or 2.5x10e4 cell equivalent-- lanes 5 and 6 respectively) were separated on 14% SDS- PAGE under reducing conditions. The separated proteins were transferred to PVDF membrane, and probed with 2G11 (1 µg/ml). AKP-conjugated polyclonal anti- mouse IgG was used as a secondary antibody at 1:1000 dilution, and the blot was developed with BCIP/NBT substrate (Sigma). The 2G11 antibody detects a single, approx. 12 kDa band protein in lanes 1 - 4 (rhuTrx) as well as well as in the cellular lysate, that corresponds to the MW of Trx. (first panel).
anti-Thioredoxin (TXN) antibody (2) Expression of Trx by human peripheral blood mononuclear cells (PBMC). Human PBMC were fixed, permeabilized, and subsequently stained with 0.125 µg of FITC-conjugated mouse anti-human Trx antibody (FITC-2G11). A histogram overlay shows specific cell staining compared to IgG1 isotype control FITC-MOPC-21 (middle panel). To demonstrate specificity of staining, the staining by FITC-2G11 was blocked by preincubation with an excess of the unlabeled 2G11 antibody (10 µg, ABIN967658) prior to staining with the FITC-2G11 antibody (second panel).
anti-Thioredoxin (TXN) antibody (3) Staining with the FITC-2G11 antibody
General Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).

Sahaf, Söderberg, Spyrou et al.: "Thioredoxin expression and localization in human cell lines: detection of full-length and truncated species." in: Experimental cell research, Vol. 236, Issue 1, pp. 181-92, 1997 (PubMed).

Bertini, Howard, Dong et al.: "Thioredoxin, a redox enzyme released in infection and inflammation, is a unique chemoattractant for neutrophils, monocytes, and T cells." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1783-9, 1999 (PubMed).

Yoshida, Katoh, Tetsuka et al.: "Involvement of thioredoxin in rheumatoid arthritis: its costimulatory roles in the TNF-alpha-induced production of IL-6 and IL-8 from cultured synovial fibroblasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 351-8, 1999 (PubMed).

Validation Images
Did you look for something else?
back to top