DDX4 antibody (DEAD Box Protein 4)

Details for Product anti-DDX4 Antibody No. ABIN967664, Supplier: Log in to see
Antigen
  • VASA
  • AV206478
  • Mvh
  • RVLG
  • vasa
  • vlg1
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • DDX4
  • Ddx4
  • ddx4
Reactivity
Human, Mouse (Murine)
97
53
29
16
6
5
4
2
1
1
1
1
1
1
1
1
Host
Human
76
21
Clonality (Clone)
Monoclonal ()
Conjugate
This DDX4 antibody is un-conjugated
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
68
36
23
23
13
13
11
7
3
2
1
1
1
1
Options
Supplier
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Brand BD Pharmingen™
Clone R11-A3
Isotype Ig Fraction, lambda
Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The recombinant Fab was purified from tissue culture supernatant by affinity chromatography.
Alternative Name VASA/DDX4 (DDX4 Antibody Abstract)
Background The R11-A3 Fab reacts with a protein of the Asp-Glu-Ala-Asp (DEAD) box (DDX) family that is homologous to the VASA protein of Drosophila. The VASA/DDX4 gene is conserved in invertebrate and vertebrate species, and its expression is germ cell specific. In the human, VASA/DDX4 is expressed in fetal and adult germ cells, both male and female, but not in other fetal and adult tissues. Studies of VASA/DDX4 expression in various types of tumors suggest that expression of VASA/DDX4 is a useful biomarker for germ cell cancers.
Synonyms: DDX4
Molecular Weight 76 kDa
Application Notes We recommend Monoclonal ANTI-FLAG® M2-Peroxidase (Sigma-Aldrich) for use as a second-step reagent for western blots and immunohistochemistry.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-DDX4 antibody (DEAD Box Protein 4) (ABIN967664) The R11-A3 Fab was selected by screening a human Fab phage display library (Hoet et a...
Western Blotting (WB) image for anti-DDX4 antibody (DEAD Box Protein 4) (ABIN967664) Western Blot validation of VASA/DDX4 by RNAi in mouse ES cell line. Lysates from ES-E...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DDX4 antibody (DEAD Box Protein 4) (ABIN967664) VASA/DDX4 staining on human (left column) and mouse (right column) testes. Following ...
Product cited in: Chu, Liu, Nix, Wu, Ralston, Yates, Meyer: "Sperm chromatin proteomics identifies evolutionarily conserved fertility factors." in: Nature, Vol. 443, Issue 7107, pp. 101-5, 2006 (PubMed).

Abdelhaleem: "RNA helicases: regulators of differentiation." in: Clinical biochemistry, Vol. 38, Issue 6, pp. 499-503, 2005 (PubMed).

Hoet, Cohen, Kent, Rookey, Schoonbroodt, Hogan, Rem, Frans, Daukandt, Pieters, van Hegelsom, Neer, Nastri, Rondon, Leeds, Hufton, Huang, Kashin, Devlin, Kuang, Steukers, Viswanathan, Nixon, Sexton et al.: "Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity. ..." in: Nature biotechnology, Vol. 23, Issue 3, pp. 344-8, 2005 (PubMed).

Clark, Bodnar, Fox, Rodriquez, Abeyta, Firpo, Pera: "Spontaneous differentiation of germ cells from human embryonic stem cells in vitro." in: Human molecular genetics, Vol. 13, Issue 7, pp. 727-39, 2004 (PubMed).

Zeeman, Stoop, Boter, Gillis, Castrillon, Oosterhuis, Looijenga: "VASA is a specific marker for both normal and malignant human germ cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 82, Issue 2, pp. 159-66, 2002 (PubMed).

Castrillon, Quade, Wang, Quigley, Crum: "The human VASA gene is specifically expressed in the germ cell lineage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 17, pp. 9585-90, 2000 (PubMed).