DEAD Box Protein 4 (DDX4) antibody

Details for Product No. ABIN967664
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Synonyms VASA, AV206478, Mvh, RVLG, vasa, vlg1
Mouse (Murine)
(71), (25), (19), (13), (1), (1), (1)
(53), (18)
Clonality (Clone)
Monoclonal ()
(3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(58), (26), (20), (16), (12), (10), (10), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Pubmed 6 references available
Quantity 0.1 mg
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Catalog No. ABIN967664
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Clone R11-A3
Isotype Ig
Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed. The conjugate should be protected from prolonged exposure to light. The antibody solution was purified from goat antiserum and was passed through solid-phase immunoadsorbent gels to minimize cross reactivity with rat, human, bovine, and horse serum proteins (multiple adsorption).
Alternative Name VASA/DDX4
Background The R11-A3 Fab reacts with a protein of the Asp-Glu-Ala-Asp (DEAD) box (DDX) family that is homologous to the VASA protein of Drosophila. The VASA/DDX4 gene is conserved in invertebrate and vertebrate species, and its expression is germ cell specific. In the human, VASA/DDX4 is expressed in fetal and adult germ cells, both male and female, but not in other fetal and adult tissues. Studies of VASA/DDX4 expression in various types of tumors suggest that expression of VASA/DDX4 is a useful biomarker for germ cell cancers.
Synonyms: DDX4
Molecular Weight 76 kDa
Application Notes We recommend Monoclonal ANTI-FLAG® M2-Peroxidase (Sigma-Aldrich) for use as a second-step reagent for western blots and immunohistochemistry.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-DEAD Box Protein 4 (DDX4) antibody The R11-A3 Fab was selected by screening a human Fab phage display library (Hoet et al.) licensed from Dyax Corp (Cambridge, MA) with immobilized recombinant human VASA/DDX4 protein. Western Blot analysis of VASA/DDX4 in mouse embryonic stem (ES) cell line. Lysate from ES-E14TG2a cells (ATCC CRL-1821) was probed with Purified Human anti-VASA/DDX4 Fab at titrations of 0.25 (lanes 1), 0.125 (lanes 2), and 0.062 µg/ml (lanes 3). VASA/DDX4 is identified as a band of 76 kDa in the mouse ES cells.
anti-DEAD Box Protein 4 (DDX4) antibody (2) Western Blot validation of VASA/DDX4 by RNAi in mouse ES cell line. Lysates from ES-E14TG2a cells (24 hour, lane 1, and 48 hour, lane 2) and VASA/DDX4 RNAi-transfected ES-E14TG2a cells (24 hour, lane 2, and 48 hour, lane 4) were probed with Purified Human anti-VASA/DDX4 Fab at 1.0 µg/ml. Down-regulation of VASA/DDX4 expression is evident in the RNAi-transfected cells. Purified Mouse anti-Actin monoclonal antibody (ABIN968902 or ABIN968903, 1:2000) was the gel-loading control.
anti-DEAD Box Protein 4 (DDX4) antibody (3) VASA/DDX4 staining on human (left column) and mouse (right column) testes. Following antigen retrieval with Retrievagen A buffer, the formalin-fixed paraffin-embedded sections were stained with either Purified Human anti-VASA/DDX4 Fab (top row) or a control Fab specific for an irrelevant antigen (bottom row), with Hematoxylin counterstaining. VASA/DDX4 is detected in spermatids and spermatocytes in the seminiferous tubules. Original magnification: 20X.
Product cited in: Chu, Liu, Nix et al.: "Sperm chromatin proteomics identifies evolutionarily conserved fertility factors." in: Nature, Vol. 443, Issue 7107, pp. 101-5, 2006 (PubMed).

Abdelhaleem: "RNA helicases: regulators of differentiation." in: Clinical biochemistry, Vol. 38, Issue 6, pp. 499-503, 2005 (PubMed).

Hoet, Cohen, Kent et al.: "Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity." in: Nature biotechnology, Vol. 23, Issue 3, pp. 344-8, 2005 (PubMed).

Clark, Bodnar, Fox et al.: "Spontaneous differentiation of germ cells from human embryonic stem cells in vitro." in: Human molecular genetics, Vol. 13, Issue 7, pp. 727-39, 2004 (PubMed).

Zeeman, Stoop, Boter et al.: "VASA is a specific marker for both normal and malignant human germ cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 82, Issue 2, pp. 159-66, 2002 (PubMed).

Castrillon, Quade, Wang et al.: "The human VASA gene is specifically expressed in the germ cell lineage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 17, pp. 9585-90, 2000 (PubMed).

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