Images for product: anti-Nanog Homeobox (NANOG) antibody
Western blot analysis of anti-mouse Nanog. Mouse embryonic stem cell lysate E14 12.5 µg/lane was probed with the Nanog monoclonal antibody at the following concentrations: 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). Nanog is identified as a 42 KDa band.
Immunofluorescent staining of mouse embryonic stem (ES) cell line. ES-E14TG2a cells (ATCC CRL-1821) were seeded in a 96-well imaging plate at ~10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Triton™ X-100, and stained with Mouse anti-Mouse Nanog antibody according to the Recommended Assay Procedure. The second step reagent was Alexa Fluor® 555 goat anti-mouse Ig (Invitrogen) (pseudo colored green). Cell nuclei were counterstained with Hoechst 33342 (pseudo colored blue). The images were captured on a BD Pathway™ 435 High-Content Bioimager System using a 20X objective and merged using BD AttoVision™ software. This antibody also stains F9 cells (mouse embryonal carcinoma, ATCC CRL-1720). It also worked with the Saponin and cold methanol fix/perm protocols, however Saponin permeabilization resulted in higher background staining.