Musashi Homolog 1 (Drosophila) (MSI1) (AA 221-311) antibody

Antigen: Musashi Homolog 1 (Drosophila) (MSI1)

Synonyms: DMSIDNA, Msi, SIDNA, anon-EST:Liang-2.35, clone 2.35, DmelCG5099, CG5099, msi1h, Musashi, MGC83965, Musashi-1, Msi1h, nrp-1B, MSI1, dsim_GLEANR_4982, musashi, DsimGD21224, GD21224, msi

Binding Site: AA 221-311

Clonality: Monoclonal (N14-47)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), BioImaging (BI), Flow Cytometry (FACS)

Catalog no.: ABIN967671

Additional Information

Alternative name: MSI1 (Musashi-1)

Immunogen: Human MSI1 a.a. 221-311 Recombinant Protein

Cross-Reactivity (Details): Predicted: Rat

Format: Liquid

Isotype: IgG1, kappa

Clone: N14-47

Description: The N14-47 monoclonal antibody recognizes RNA-binding protein Musashi homolog 1 (Musashi-1 or MSI1) that represses the translation of particular mRNAs by binding specific RNA sequence motifs. MSI1 contains two RNA-recognition domains at amino acids 20-110 and 109-186. It is expressed in early lineage cells, such as neural stem/progenitor cells, small intestinal stem cells, and mammary stem cells. MSI1 has also been detected in many human cancers, including gliomas, melanomas, colorectal adenomas and adenocarcinomas, suggesting its involvement in cancer development. Western blot analysis and immunofluorescent staining of MSI1 in neural stem cells (NSC) derived from H9 human embryonic stem cells (WiCell, Madison, WI).
Synonyms: Musashi-1, RNA-binding protein Musashi homolog 1, MSI1H

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Triton is a trademark of the Dow Chemical Company.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Molecular Weight: 39 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

NSC lysate was probed with Purified Mouse anti-Human MSI1 monoclonal antibody at concentrations of 1.0, 0.5, and 0.25 µg/ml (lanes 1, 2, and 3, respectively). MSI1 is identified as a band of 39 kDa.

NSC were fixed with BD Cytofix™ fixation buffer, permeabilized with 0.1% Triton™-X 100, and stained with Purified Mouse anti-Human MSI1 monoclonal antibody (pseudo-colored green) at 5 myg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies) and counter-staining was with Hoechst 33342 (pseudo-colored blue). The image was captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software. The staining worked with the cold methanol (BD™ Phosflow Perm Buffer III) and the Triton X-100 Perm/Wash protocols, but not with BD™ Phosflow Perm/Wash Buffer I.

Publications

Product: Okano, Imai, Okabe: "Musashi: a translational regulator of cell fate." in: Journal of cell science, Vol. 115, Issue Pt 7, pp. 1355-9, 2002 (PubMed).

Montgomery, Breault: "Small intestinal stem cell markers." in: Journal of anatomy, Vol. 213, Issue 1, pp. 52-8, 2008 (PubMed).

Glazer, Wang, Yuan et al.: "Musashi1: a stem cell marker no longer in search of a function." in: Cell cycle (Georgetown, Tex.), Vol. 7, Issue 17, pp. 2635-9, 2008 (PubMed).