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OCT3/4A (Isoform A) antibody

Reactivity: Human, Mouse BI, IF, ICS, WB Host: Mouse Monoclonal O50-808 unconjugated
Catalog No. ABIN967679
  • Target
    OCT3/4A
    Binding Specificity
    Isoform A
    Reactivity
    Human, Mouse
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    BioImaging (BI), Immunofluorescence (IF), Intracellular Staining (ICS), Western Blotting (WB)
    Brand
    BD Pharmingen™
    Cross-Reactivity
    Mouse (Murine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Oct3/4 Isoform A Recombinant Protein
    Clone
    O50-808
    Isotype
    IgG1 kappa
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Pan, Thomson: "Nanog and transcriptional networks in embryonic stem cell pluripotency." in: Cell research, Vol. 17, Issue 1, pp. 42-9, (2007) (PubMed).

    Nishimoto, Fukushima, Okuda, Muramatsu: "The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2." in: Molecular and cellular biology, Vol. 19, Issue 8, pp. 5453-65, (1999) (PubMed).

    Vigano, Staudt: "Transcriptional activation by Oct-3: evidence for a specific role of the POU-specific domain in mediating functional interaction with Oct-1." in: Nucleic acids research, Vol. 24, Issue 11, pp. 2112-8, (1996) (PubMed).

    Yuan, Corbi, Basilico, Dailey: "Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3." in: Genes & development, Vol. 9, Issue 21, pp. 2635-45, (1995) (PubMed).

    Rosfjord, Scholtz, Lewis, Rizzino: "Phosphorylation and DNA binding of the octamer binding transcription factor Oct-3." in: Biochemical and biophysical research communications, Vol. 212, Issue 3, pp. 847-53, (1995) (PubMed).

    Okamoto, Okazawa, Okuda, Sakai, Muramatsu, Hamada: "A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells." in: Cell, Vol. 60, Issue 3, pp. 461-72, (1990) (PubMed).

  • Target
    OCT3/4A
    Background
    Development of a multicellular organism from a single fertilized egg is regulated by the coordinated activity of DNA transcription factors. Oct3/4, a member of the POU family of transcription factors, functions in pluripotent cells of early embryonic stem (ES) cell lines and embryonal carcinomas (EC). The human POU5F1 gene can encode various splice variants, two of which are Oct3/4A and Oct3/4B. Both isoforms share identical POU DNA-binding and C-terminal domains but differ in their N-terminal domain. The N-terminal domain of Oct3/4B is inhibitory to the DNA binding domain and therefore cannot stimulate transcription of Oct3/4-dependent genes. Oct3/4B can be detected in both pluripotent and some differentiated cell types in both the nucleus and cytoplasm, but its function is unclear. There is not an equivalent to Oct3/4B in mouse. Oct3/4A is expressed in the nucleus and has been demonstrated to orchestrate the transcription of Oct3/4-dependent genes. It has been demonstrated that the expression of Oct3/4 isoforms can vary greatly in different cell types, and discrimination of these is crucial for assessing Oct3/4 expression and function. The O50-808 monoclonal antibody recognizes human Oct3/4 Isoform A and mouse Oct3/4.
    Synonyms: Oct3/4A, Oct-3A, OTF-3, NF-A3, OTF4, POU5F1, MGC22487
    Molecular Weight
    46 kDa
    Gene ID
    5460, 18999
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