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Phosphotyrosine antibody

WB, IP, IF, IHC Host: Mouse Monoclonal PY20 unconjugated
Catalog No. ABIN967685
  • Target See all Phosphotyrosine products
    Phosphotyrosine
    Reactivity
    Please inquire
    Host
    • 90
    • 28
    • 1
    Mouse
    Clonality
    • 102
    • 17
    Monoclonal
    Conjugate
    • 42
    • 16
    • 10
    • 8
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Phosphotyrosine antibody is un-conjugated
    Application
    • 85
    • 61
    • 40
    • 38
    • 37
    • 28
    • 23
    • 17
    • 13
    • 9
    • 9
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Clone
    PY20
    Isotype
    IgG2b
  • Application Notes
    The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis. Other applications were tested during antibody development only or reported in the literature.
    Comment

    Related Products: ABIN968534, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    Aqueous buffered solution containing glycerol and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Yoon, Kovalenko, Bogdanov, Wallach: "MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation." in: Immunity, Vol. 47, Issue 1, pp. 51-65.e7, (2017) (PubMed).

    Yamakawa, Tsuchida, Sugino: "The rasGAP-binding protein, Dok-1, mediates activin signaling via serine/threonine kinase receptors." in: The EMBO journal, Vol. 21, Issue 7, pp. 1684-94, (2002) (PubMed).

    Baisden, Gatesman, Cherezova, Jiang, Flynn: "The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases." in: Oncogene, Vol. 20, Issue 45, pp. 6607-16, (2001) (PubMed).

    Lund-Johansen, Davis, Bishop, de Waal Malefyt: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." in: Cytometry, Vol. 39, Issue 4, pp. 250-9, (2000) (PubMed).

    Vinós, Freeman: "Evidence that Argos is an antagonistic ligand of the EGF receptor." in: Oncogene, Vol. 19, Issue 31, pp. 3560-2, (2000) (PubMed).

    Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, (1988) (PubMed).

  • Target
    Phosphotyrosine
    Abstract
    Phosphotyrosine Products
    Target Type
    Amino Acid
    Background
    Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins. Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis.
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