Phosphotyrosine antibody (Biotin)

Antigen: Phosphotyrosine

Clonality: Monoclonal (PY20)

Host: Mouse

Reactivity: Human, Chicken, Dog (Canine), Frog, Mouse (Murine), Rat (Rattus)

Conjugate: Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN967691

Additional Information

Format: Liquid

Isotype: IgG2b

Clone: PY20

Description: Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins. Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Comments:

Related Products: ABIN968534, ABIN967389

Application Details

Application Notes: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis. Other applications were tested during antibody development only or reported in the literature.

Concentration: 250 µg/ml

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Metabolism, Amino Acids, Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of phosphotyrosine on A431 lysate. Lane 1: 1:2000, lane 2: 1:4000, lane 3: 1:8000 dilution of anti-phosphotyrosine, PY20.

Publications

Product: Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).

Arvidsson, Rupp, Nånberg et al.: "Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation." in: Molecular and cellular biology, Vol. 14, Issue 10, pp. 6715-26, 1994 (PubMed).

Nishikawa, Ji, Harmon et al.: "A mutant epidermal growth factor receptor common in human glioma confers enhanced tumorigenicity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 16, pp. 7727-31, 1994 (PubMed).

Fan, Mendelsohn, Masui et al.: "Regulation of epidermal growth factor receptor in NIH3T3/HER14 cells by antireceptor monoclonal antibodies." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21073-9, 1993 (PubMed).

Kuppuswamy, Kerr, Narishige et al.: "Association of tyrosine-phosphorylated c-Src with the cytoskeleton of hypertrophying myocardium." in: The Journal of biological chemistry, Vol. 272, Issue 7, pp. 4500-8, 1997 (PubMed).