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Phosphotyrosine antibody (Biotin)

Details for Product No. ABIN967690, Supplier: Log in to see
Antigen
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
107
50
46
29
27
26
24
8
8
6
1
Host
Mouse
178
63
2
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
26
24
17
14
7
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
179
109
85
78
72
54
27
25
11
7
7
2
1
1
1
1
1
Supplier
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Clone PY20
Isotype IgG2b
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins. Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis.
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications
Application Notes The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis. Other applications were tested during antibody development only or reported in the literature.
Comment

Related Products: ABIN968534, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol.
Storage -20 °C
Storage Comment The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Phosphotyrosine antibody (Biotin) (ABIN967690) Western blot analysis of phosphotyrosine on A431 lysate. Lane 1: 1:2000, lane 2: 1:40...
Product cited in: Kuppuswamy, Kerr, Narishige, Kasi, Menick, Cooper: "Association of tyrosine-phosphorylated c-Src with the cytoskeleton of hypertrophying myocardium." in: The Journal of biological chemistry, Vol. 272, Issue 7, pp. 4500-8, 1997 (PubMed).

Arvidsson, Rupp, Nånberg, Downward, Rönnstrand, Wennström, Schlessinger, Heldin, Claesson-Welsh: "Tyr-716 in the platelet-derived growth factor beta-receptor kinase insert is involved in GRB2 binding and Ras activation." in: Molecular and cellular biology, Vol. 14, Issue 10, pp. 6715-26, 1994 (PubMed).

Nishikawa, Ji, Harmon, Lazar, Gill, Cavenee, Huang: "A mutant epidermal growth factor receptor common in human glioma confers enhanced tumorigenicity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 16, pp. 7727-31, 1994 (PubMed).

Fan, Mendelsohn, Masui, Kumar: "Regulation of epidermal growth factor receptor in NIH3T3/HER14 cells by antireceptor monoclonal antibodies." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21073-9, 1993 (PubMed).

Background publications Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).