Phosphotyrosine antibody

Antigen: Phosphotyrosine

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Chicken, Dog (Canine), Frog, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN967692

Additional Information

Format: Liquid

Isotype: Ig

Description: Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine are excellent tools for the detection and characterization of phosphotyrosine-containing proteins. Polyclonal antibodies can offer advantages over monoclonals in certain immunoprecipitation protocols and can, theoretically, react with a broader range of phosphotyrosine-containing proteins.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Application Details

Application Notes: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from antiserum by negative adsorption and affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Metabolism, Amino Acids, Protein Modifications

Restrictions: For Research Use only

Images

Western blot analysis of Phosphotyrosine on A431+EGF lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of Phosphotyrosine.

A431 control (First Panel) and A431 + EGF (Second Panel).

Publications

Product: Wang: "Antibodies for phosphotyrosine: analytical and preparative tool for tyrosyl-phosphorylated proteins." in: Analytical biochemistry, Vol. 172, Issue 1, pp. 1-7, 1988 (PubMed).

Ross, Baltimore, Eisen: "Phosphotyrosine-containing proteins isolated by affinity chromatography with antibodies to a synthetic hapten." in: Nature, Vol. 294, Issue 5842, pp. 654-6, 1982 (PubMed).

Schoenwaelder, Burridge: "Evidence for a calpeptin-sensitive protein-tyrosine phosphatase upstream of the small GTPase Rho. A novel role for the calpain inhibitor calpeptin in the inhibition of protein-tyrosine phosphatases." in: The Journal of biological chemistry, Vol. 274, Issue 20, pp. 14359-67, 1999 (PubMed).

Keilhack, Müller, Böhmer et al.: "Negative regulation of Ros receptor tyrosine kinase signaling. An epithelial function of the SH2 domain protein tyrosine phosphatase SHP-1." in: The Journal of cell biology, Vol. 152, Issue 2, pp. 325-34, 2001 (PubMed).

Byron, Lucchesi: "Signal transduction of physiological concentrations of vasopressin in A7r5 vascular smooth muscle cells. A role for PYK2 and tyrosine phosphorylation of K+ channels in the stimulation of Ca2+ spiking." in: The Journal of biological chemistry, Vol. 277, Issue 9, pp. 7298-307, 2002 (PubMed).