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Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 325-345) antibody

Antigen

Mitogen-Activated Protein Kinase 3 (MAPK3)
Synonyms ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180, zERK1, fi06b09, wu:fi06b09, Tb08.10J17.940
Binding Site
Alternatives

AA 325-345
Clonality Monoclonal (MK12)
Host
Alternatives

Mouse
Reactivity
Alternatives

Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
5 references available
Catalog no. ABIN967700
Quantity 50 µg  (250 µg/ml)  (Variants)
Price Product not available in this region.
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anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 325-345) antibody

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 325-345) antibody (2)

anti-Mitogen-Activated Protein Kinase 3 (MAPK3) (AA 325-345) antibody (3)

Additional Information
Application Details Publications Images Alternatives Variants back to top

Alternative name ERK1
Immunogen Rat ERK1
Cross-Reactivity Mouse (Murine), Human, Chicken, Dog (Canine), Frog
Format Liquid
Isotype IgG1
Clone MK12
Description The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) is activated after cell stimulation by a variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. A myriad of proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphatases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK1 is a 44 kDa member of the ERK family and shares 85% homology with ERK2 (42 kDa).
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Molecular Weight 44 kDa
Comments

Related Products: ABIN968545, ABIN967389

Application Details
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Concentration 250 µg/ml
Purity Purified
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative 0.09% Sodium azide.
Storage Store undiluted at -20° C.
Research Area Stem Cells, Alzheimer's Disease, Signaling, Inflammation
Restrictions For Research Use only

Publications
Application Details Images Alternatives Variants back to top

Product Reszka, Seger, Diltz et al.: "Association of mitogen-activated protein kinase with the microtubule cytoskeleton." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 19, pp. 8881-5, 1995 (PubMed).

Wan, Huang: "Analysis of the Gs/mitogen-activated protein kinase pathway in mutant S49 cells." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14533-7, 1998 (PubMed).

Ackerley, Grierson, Brownlees et al.: "Glutamate slows axonal transport of neurofilaments in transfected neurons." in: The Journal of cell biology, Vol. 150, Issue 1, pp. 165-76, 2000 (PubMed).

Aguirre-Ghiso, Liu, Mignatti et al.: "Urokinase receptor and fibronectin regulate the ERK(MAPK) to p38(MAPK) activity ratios that determine carcinoma cell proliferation or dormancy in vivo." in: Molecular biology of the cell, Vol. 12, Issue 4, pp. 863-79, 2001 (PubMed).

Nowak: "Protein kinase C-alpha and ERK1/2 mediate mitochondrial dysfunction, decreases in active Na+ transport, and cisplatin-induced apoptosis in renal cells." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 43377-88, 2002 (PubMed).

Alternatives
Application Details Publications Images Variants back to top

Alternatives for antigen "Mitogen-Activated Protein Kinase 3 (MAPK3)", type "Antibodies"
Hosts Rabbit (218), Mouse (30), Goat (7), Chicken (1), Sheep (1)
Reactivities Human (242), Rat (Rattus) (159), Mouse (Murine) (143), Cow (Bovine) (61), Dog (Canine) (59), Chicken (58), Pig (Porcine) (55), Guinea Pig (54), Horse (Equine) (54), Xenopus laevis (5), Fruit Fly (Drosophila melanogaster) (2), Monkey (2), Rabbit (2), Sheep (Ovine) (2), Cat (Feline) (1), Primate (1), Zebrafish (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (163), ELISA (96), Immunofluorescence (IF) (92), Immunohistochemistry (IHC) (46), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (44), Immunoprecipitation (IP) (34), Immunocytochemistry (ICC) (14), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Flow Cytometry (FACS) (6), Dot Blot (Dot) (4), Immunoelectron Microscopy (IEM) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Dot Blot (DB) (2), Functional Studies (Func) (1)
Conjugates Biotin (7), FITC (7), HRP (5), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), Gold (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
Epitopes C-Term (34), pTyr204 (30), pThr202,pTyr204 (18), pThr197,pThr202 (13), N-Term (12), pThr202 (10), Internal Region (8), pThr202, pTyr204 (8), Tyr204 (5), pThr202,pThr197 (5), pTyr204,pThr202 (5), C-Term,AA 365-379 (4), Thr202 (4), pThr202, pThr204 (4), pThr-Glu-pTyr (3), Asp192 (2), pThr185, pTyr187 (2), AA 174-183 (1), AA 324-345 (1), AA 34-0 (1), AA 350-400 (1), C-Term,AA 352-380 (1), Tyr202 (1), pT-E-pY Motif (1)