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Cortactin antibody (CTTN)

Details for Product anti-CTTN Antibody No. ABIN967710, Supplier: Log in to see
Antigen
  • 1110020L01Rik
  • CG3637
  • cortactin
  • Cttn
  • CTTN
  • cttn
  • CTTN1
  • Cttnb
  • Dmel\CG3637
  • EMS1
  • Ems1
  • ems1
  • P85.25
Alternatives
anti-Human Cortactin antibody for ELISA
Reactivity
Dog (Canine), Human
179
106
60
7
7
5
3
2
2
1
1
1
1
1
1
Host
Mouse
166
24
15
Clonality (Clone)
Monoclonal ()
Conjugate
This Cortactin antibody is un-conjugated
4
4
4
4
4
4
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
170
89
85
22
15
8
7
3
3
1
1
1
1
Supplier
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Immunogen Chicken Cortactin
Clone 30-Cortactin
Isotype IgG1
Cross-Reactivity Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cortactin (CTTN Antibody Abstract)
Background Cortactin is a protein which is represented by two structurally related isoforms of 80 kDa and 85 kDa. Sequence analysis of this protein reveals the presence of a carboxy-terminal SH3 domain, as well as six internal tandem repeats of a 37 amino acid sequence. It is able to bind to F-actin via this N-terminal repeat region, and colocalizes with actin in the cell. Cortactin was originally described as a protein that is tyrosine-phosphorylated in cells transformed by the src oncogene, in which it localizes to rosettes or podosomes. It has since been shown that other stimuli, such as bacterial invasion, treatment with growth factors, and integrin-mediated cell adhesion, are also capable of inducing phosphorylation of cortactin. Some additional evidence indicates that cortactin may actually be a substrate of syk, with src acting as an upstream signaling molecule. This antibody is routinely tested by western blot analysis.
Molecular Weight 85/80 kDa
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments
Pathways MAPK Signaling
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Cortactin antibody (CTTN) (ABIN967710) Western blot analysis of Cortactin on a A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, ...
Immunofluorescence (IF) image for anti-Cortactin antibody (CTTN) (ABIN967710) Immunofluoresence staining of NIH-3T3 cells.
 image for anti-Cortactin antibody (CTTN) (ABIN967710) anti-Cortactin (CTTN) antibody (Image 3)
Western Blotting (WB) image for anti-Cortactin antibody (CTTN) (ABIN967710) anti-Cortactin (CTTN) antibody (Image 4)
Product cited in: Cantarelli, Takahashi, Yanagihara, Akeda, Imura, Kodama, Kono, Sato, Iida, Honda: "Cortactin is necessary for F-actin accumulation in pedestal structures induced by enteropathogenic Escherichia coli infection." in: Infection and immunity, Vol. 70, Issue 4, pp. 2206-9, 2002

Lavoie, Champagne, Gingras, Robert: "Adenovirus E4 open reading frame 4-induced apoptosis involves dysregulation of Src family kinases." in: The Journal of cell biology, Vol. 150, Issue 5, pp. 1037-56, 2000

Nakahara, Mueller, Nomizu, Yamada, Yeh, Chen: "Activation of beta1 integrin signaling stimulates tyrosine phosphorylation of p190RhoGAP and membrane-protrusive activities at invadopodia." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 9-12, 1998

Dehio, Prévost, Sansonetti: "Invasion of epithelial cells by Shigella flexneri induces tyrosine phosphorylation of cortactin by a pp60c-src-mediated signalling pathway." in: The EMBO journal, Vol. 14, Issue 11, pp. 2471-82, 1995

Wu, Parsons: "Cortactin, an 80/85-kilodalton pp60src substrate, is a filamentous actin-binding protein enriched in the cell cortex." in: The Journal of cell biology, Vol. 120, Issue 6, pp. 1417-26, 1993

Wu, Reynolds, Kanner, Vines, Parsons: "Identification and characterization of a novel cytoskeleton-associated pp60src substrate." in: Molecular and cellular biology, Vol. 11, Issue 10, pp. 5113-24, 1991