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Cortactin (CTTN) antibody

Details for Product No. ABIN967710
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Antigen
Synonyms Cttn, CTTN, cttn, ems1, CG3637, Dmel\CG3637, cortactin, Ems1, 1110020L01Rik, CTTN1, P85.25, EMS1, Cttnb
Reactivity
Chicken
(149), (93), (56), (16), (15), (14), (1), (1), (1)
Host
Mouse
(151), (17), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
(140), (69), (60), (27), (20), (7), (3), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 150 µg
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Catalog No. ABIN967710
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Immunogen Chicken Cortactin
Clone OPG-13
Isotype IgG1
Cross-Reactivity Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Cortactin
Background Cortactin is a protein which is represented by two structurally related isoforms of 80 kDa and 85 kDa. Sequence analysis of this protein reveals the presence of a carboxy-terminal SH3 domain, as well as six internal tandem repeats of a 37 amino acid sequence. It is able to bind to F-actin via this N-terminal repeat region, and colocalizes with actin in the cell. Cortactin was originally described as a protein that is tyrosine-phosphorylated in cells transformed by the src oncogene, in which it localizes to rosettes or podosomes. It has since been shown that other stimuli, such as bacterial invasion, treatment with growth factors, and integrin-mediated cell adhesion, are also capable of inducing phosphorylation of cortactin. Some additional evidence indicates that cortactin may actually be a substrate of syk, with src acting as an upstream signaling molecule. This antibody is routinely tested by western blot analysis.
Molecular Weight 85/80 kDa
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Cortactin (CTTN) antibody Western blot analysis of Cortactin on a A431 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- Cortactin antibody.
anti-Cortactin (CTTN) antibody (2) Immunofluoresence staining of NIH-3T3 cells.
anti-Cortactin (CTTN) antibody (3) anti-Cortactin (CTTN) antibody (Image 3)
Product cited in: Wu, Reynolds, Kanner et al.: "Identification and characterization of a novel cytoskeleton-associated pp60src substrate." in: Molecular and cellular biology, Vol. 11, Issue 10, pp. 5113-24, 1991 (PubMed).

Dehio, Prévost, Sansonetti: "Invasion of epithelial cells by Shigella flexneri induces tyrosine phosphorylation of cortactin by a pp60c-src-mediated signalling pathway." in: The EMBO journal, Vol. 14, Issue 11, pp. 2471-82, 1995 (PubMed).

Wu, Parsons: "Cortactin, an 80/85-kilodalton pp60src substrate, is a filamentous actin-binding protein enriched in the cell cortex." in: The Journal of cell biology, Vol. 120, Issue 6, pp. 1417-26, 1993 (PubMed).

Nakahara, Mueller, Nomizu et al.: "Activation of beta1 integrin signaling stimulates tyrosine phosphorylation of p190RhoGAP and membrane-protrusive activities at invadopodia." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 9-12, 1998 (PubMed).

Lavoie, Champagne, Gingras et al.: "Adenovirus E4 open reading frame 4-induced apoptosis involves dysregulation of Src family kinases." in: The Journal of cell biology, Vol. 150, Issue 5, pp. 1037-56, 2000 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967710
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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