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Paxillin antibody (PXN) (AA 1-557)

Details for Product anti-PXN Antibody No. ABIN967712, Supplier: Login to see New
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Antigen
Synonyms CG18061, CG18576, CG31794, CT40481, CT42454, DPaxillin, DPxn, DPxn37, Dmel\\CG31794, Dpax, DpaxA, PDLP, dPax, pax, PXN, LOC100220858, AW108311, AW123232, Pax, wu:fw71f12
Alternatives
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Epitope
AA 1-557
(62), (53), (28), (24), (23), (18), (14), (13), (9), (9), (9), (8), (7), (7), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(385), (165), (151), (26), (5), (3), (3), (2), (1), (1), (1), (1), (1)
Host
Mouse
(352), (50), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
This Paxillin antibody is un-conjugated
(7), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (1), (1)
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(311), (106), (92), (58), (55), (53), (30), (30), (8), (8), (3), (2), (2), (2), (1), (1)
Pubmed 5 references available
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Quantity 150 μg
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Immunogen Chicken Paxillin aa. 1-557
Clone 349-Paxillin
Isotype IgG1
Cross-Reactivity Human, Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Paxillin (PXN Antibody Abstract)
Background A number of cytoskeletal proteins are tyrosine phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts. One of these is the 68 kDa paxillin protein. Paxillin is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers. It is also present in the focal adhesions of Madin-Darby canine kidney epithelial cells, but is absent from the cell adherens junctions of these cells. Paxillin purified from chicken gizzard migrates as a diffuse band on SDS-PAGE with molecular weight of 65-70 kDa. It binds to the rod domain of vinculin, another focal adhesion protein. It is thought that phosphorylation of paxillin may have a role in that disassembly of focal adhesions and stress fibers during transformation. This antibody is routinely tested by Western blot analysis.
Molecular Weight 68 kDa
Research Area Cancer, Extracellular Matrix
Pathways MAPK Signaling, EGFR Signaling Pathway
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Paxillin antibody (PXN) (AA 1-557) (ABIN967712) Western blot analysis of Paxillin on a A431 lysate. Lane 1: 1:10,000, lane 2: 1:20,00...
Immunofluorescence (IF) image for anti-Paxillin antibody (PXN) (AA 1-557) (ABIN967712) Immunofluoresence staining of human fibroblasts.
Product cited in: van de Water, Houtepen, Huigsloot et al.: "Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial (LLC-PK1) cells by focal adhesion kinase (FAK). Role of FAK in maintaining focal adhesion organization after acute renal cell injury." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36183-93, 2001 (PubMed).

Even-Ram, Maoz, Pokroy et al.: "Tumor cell invasion is promoted by activation of protease activated receptor-1 in cooperation with the alpha vbeta 5 integrin." in: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 10952-62, 2001 (PubMed).

Nikolopoulos, Turner: "Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion." in: The Journal of cell biology, Vol. 151, Issue 7, pp. 1435-48, 2001 (PubMed).

Wine, Chapin: "Adhesion and signaling proteins spatiotemporally associated with spermiation in the rat." in: Journal of andrology, Vol. 20, Issue 2, pp. 198-213, 1999 (PubMed).

Background publications Glenney, Zokas: "Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton." in: The Journal of cell biology, Vol. 108, Issue 6, pp. 2401-8, 1989 (PubMed).

Catalog No. ABIN967712
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