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GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 252-270)

Details for Product anti-GJA1 Antibody No. ABIN967718, Supplier: Login to see New
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Antigen
Synonyms
cx43, gjal, oddd, dfnb38, connexin43, cx43a1, gja1, zfCx43.3, AU042049, AW546267, Cnx43, Cx43, Cx43alpha1, Gja-1, Npm1, AVSD3, CX43, DFNB38, GJAL, HLHS1, HSS, ODDD, connexin 43, gja1-A, Cnx46, Cx46, G ... show more
cx43, gjal, oddd, dfnb38, connexin43, cx43a1, gja1, zfCx43.3, AU042049, AW546267, Cnx43, Cx43, Cx43alpha1, Gja-1, Npm1, AVSD3, CX43, DFNB38, GJAL, HLHS1, HSS, ODDD, connexin 43, gja1-A, Cnx46, Cx46, Gja-3, AI118175, Cnx32, Cx32, Gjb-1 show less
Alternatives
Epitope
AA 252-270
(62), (23), (21), (19), (13), (13), (11), (10), (10), (8), (7), (7), (6), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(239), (166), (140), (42), (19), (14), (12), (11), (10), (9), (9), (7), (6), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host
Mouse
(256), (21), (21)
Clonality (Clone)
Monoclonal ()
Conjugate
This GJA1 antibody is un-conjugated
(6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
(231), (111), (103), (66), (40), (20), (14), (13), (8), (6), (3), (2), (2), (1)
Pubmed 5 references available
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Quantity 150 μg
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Immunogen Rat Connexin-43 aa. 252-270
Clone 2-Connexin
Isotype IgG1
Cross-Reactivity Chicken, Dog (Canine), Human, Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Connexin-43 (GJA1 Antibody Abstract)
Background Gap junctions are intercellular protein pores. They enable cell-to-cell communication by allowing passage of ions and other small molecules. The subunits of gap junction channels are assembled from a family of proteins called connexins. Individual connexin molecules join to make hexameric hemichannels termed connexons, these structures dock to connexons on neighboring cells, forming gap junction pores. Connexin-43 is a member of the connexin family possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. In addition, it is believed that phosphorylation of connexin-43 plays a regulatory role both in the assembly of connexons and in gating activity at the gap junction. This antibody is routinely tested by western blot analysis.
Molecular Weight 43 kDa
Research Area Extracellular Matrix, Cytoskeleton, Signaling
Pathways MAPK Signaling
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 252-270) (ABIN967718) Western blot analysis of Connexin-43 on a rat cerebrum lysate. Lane 1: 1:250, lane 2:...
Immunofluorescence (IF) image for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 252-270) (ABIN967718) Immunofluorescence staining on WI38 cells (human lung).
Image no. 3 for anti-GJA1 antibody (Gap Junction Protein, alpha 1, 43kDa) (AA 252-270) (ABIN967718) anti-Gap Junction Protein, alpha 1, 43kDa (GJA1) (AA 252-270) antibody (Image 3)
Product cited in: Giepmans, Hengeveld, Postma et al.: "Interaction of c-Src with gap junction protein connexin-43. Role in the regulation of cell-cell communication." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8544-9, 2001 (PubMed).

Toyofuku, Akamatsu, Zhang et al.: "c-Src regulates the interaction between connexin-43 and ZO-1 in cardiac myocytes." in: The Journal of biological chemistry, Vol. 276, Issue 3, pp. 1780-8, 2001 (PubMed).

Lucke, Choudhry, Thom et al.: "Upregulation of connexin 26 is a feature of keratinocyte differentiation in hyperproliferative epidermis, vaginal epithelium, and buccal epithelium." in: The Journal of investigative dermatology, Vol. 112, Issue 3, pp. 354-61, 1999 (PubMed).

Laird, Castillo, Kasprzak: "Gap junction turnover, intracellular trafficking, and phosphorylation of connexin43 in brefeldin A-treated rat mammary tumor cells." in: The Journal of cell biology, Vol. 131, Issue 5, pp. 1193-203, 1996 (PubMed).

Loo, Berestecky, Kanemitsu et al.: "pp60src-mediated phosphorylation of connexin 43, a gap junction protein." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12751-61, 1995 (PubMed).

Catalog No. ABIN967718
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