You are viewing an incomplete version of our website. Please click to reload the website as full version.

FAK antibody (PTK2 Protein tyrosine Kinase 2) (AA 354-533)

Details for Product anti-PTK2 Antibody No. ABIN967736, Supplier: Log in to see
Antigen
  • FADK
  • FAK
  • FAK1
  • FRNK
  • PPP1R71
  • p125FAK
  • pp125FAK
  • Fadk
  • mKIAA4203
Epitope
AA 354-533
67
59
57
48
45
32
22
22
20
15
14
14
13
13
13
12
9
9
8
7
7
7
7
6
5
5
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
613
357
330
20
5
5
3
3
2
2
2
1
1
1
Host
Mouse
556
90
2
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This FAK antibody is un-conjugated
17
16
15
13
12
12
11
11
11
11
11
11
3
2
2
1
Application
BioImaging (BI), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
442
181
154
124
97
82
50
18
12
7
5
5
3
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen Chicken FAK aa. 354-533
Clone 77-FAK
Isotype IgG1
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name FAK (PTK2 Antibody Abstract)
Background Focal Adhesion Kinase (FAK) is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the Focal Adhesion Targeting sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation and activation of FAK. This creates binding sites for SH2 domains of intracellular signaling molecules such as src, PI3 kinase, and Grb2. FAK's ability to bind numerous structural and signaling proteins via a variety of interactions has led to substantial speculation about its function. Although FAK's precise role has not been elucidated, proposed possibilities include regulating cell motility, cell growth, cytoskeletal organization, and adhesion-dependent cell survival.
Synonyms: Focal Adhesion Kinase
Molecular Weight 116-125 kDa
Research Area Cancer, Extracellular Matrix, Tyrosine Kinases, Angiogenesis, Kinases/Phosphatases
Application Notes Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.
Comment

Related Products: ABIN967389, ABIN968533

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-FAK antibody (PTK2 Protein tyrosine Kinase 2) (AA 354-533) (ABIN967736) Western blot analysis of FAK on a A431 cell lysate (Human epithelial carcinoma, ATCC ...
Immunofluorescence (IF) image for anti-FAK antibody (PTK2 Protein tyrosine Kinase 2) (AA 354-533) (ABIN967736) Immunofluorescent staining of U2OS (ATCC HTB-96) cells. Cells were seeded in a 96-wel...
Product cited in: Yi, Zhou, Huber et al.: "Nuclear compartmentalization of FAK and FRNK in cardiac myocytes." in: American journal of physiology. Heart and circulatory physiology, Vol. 290, Issue 6, pp. H2509-15, 2006 (PubMed).

Jones, Stewart: "Nuclear import of N-terminal FAK by activation of the FcepsilonRI receptor in RBL-2H3 cells." in: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 39-45, 2004 (PubMed).

Zeng, Si, Yu et al.: "PTP alpha regulates integrin-stimulated FAK autophosphorylation and cytoskeletal rearrangement in cell spreading and migration." in: The Journal of cell biology, Vol. 160, Issue 1, pp. 137-46, 2003 (PubMed).

Kim, Feldman: "Insulin-like growth factor I prevents mannitol-induced degradation of focal adhesion kinase and Akt." in: The Journal of biological chemistry, Vol. 277, Issue 30, pp. 27393-400, 2002 (PubMed).

Kovacic-Milivojević, Roediger, Almeida et al.: "Focal adhesion kinase and p130Cas mediate both sarcomeric organization and activation of genes associated with cardiac myocyte hypertrophy." in: Molecular biology of the cell, Vol. 12, Issue 8, pp. 2290-307, 2001 (PubMed).

Huan, van Adelsberg: "Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins." in: The Journal of clinical investigation, Vol. 104, Issue 10, pp. 1459-68, 1999 (PubMed).

Clancy, Rediske, Tang et al.: "Outside-in signaling in the chondrocyte. Nitric oxide disrupts fibronectin-induced assembly of a subplasmalemmal actin/rho A/focal adhesion kinase signaling complex." in: The Journal of clinical investigation, Vol. 100, Issue 7, pp. 1789-96, 1997 (PubMed).