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FAK antibody (AA 354-533)

PTK2 Reactivity: Human, Mouse, Rat, Chicken, Dog WB, IHC, IP, BI Host: Mouse Monoclonal 77-FAK unconjugated
Catalog No. ABIN967736
  • Target See all FAK (PTK2) Antibodies
    FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
    Binding Specificity
    • 37
    • 29
    • 27
    • 25
    • 23
    • 23
    • 18
    • 18
    • 16
    • 16
    • 13
    • 11
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 354-533
    Reactivity
    • 326
    • 285
    • 220
    • 22
    • 21
    • 18
    • 17
    • 12
    • 9
    • 7
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Dog
    Host
    • 338
    • 18
    • 3
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 328
    • 35
    Monoclonal
    Conjugate
    • 179
    • 19
    • 16
    • 13
    • 13
    • 13
    • 13
    • 13
    • 13
    • 13
    • 10
    • 9
    • 9
    • 9
    • 9
    • 9
    • 2
    • 1
    This FAK antibody is un-conjugated
    Application
    • 299
    • 126
    • 122
    • 121
    • 108
    • 75
    • 68
    • 52
    • 31
    • 30
    • 25
    • 5
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), BioImaging (BI)
    Cross-Reactivity
    Human, Mouse (Murine), Rat (Rattus), Dog (Canine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    6. Triton is a trademark of the Dow Chemical Company.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Chicken FAK aa. 354-533
    Clone
    77-FAK
    Isotype
    IgG1
    Top Product
    Discover our top product PTK2 Primary Antibody
  • Application Notes
    Bioimaging
    1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
    2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
    3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
    4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
    5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
    6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
    7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
    8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
    9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
    10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
    11. View and analyze the cells on an appropriate imaging instrument.
    Comment

    Related Products: ABIN967389, ABIN968533

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Yi, Zhou, Huber, Qu, Wang, Gerdes, Li: "Nuclear compartmentalization of FAK and FRNK in cardiac myocytes." in: American journal of physiology. Heart and circulatory physiology, Vol. 290, Issue 6, pp. H2509-15, (2006) (PubMed).

    Jones, Stewart: "Nuclear import of N-terminal FAK by activation of the FcepsilonRI receptor in RBL-2H3 cells." in: Biochemical and biophysical research communications, Vol. 314, Issue 1, pp. 39-45, (2004) (PubMed).

    Zeng, Si, Yu, Le, Ng, Teng, Ryan, Wang, Ponniah, Pallen: "PTP alpha regulates integrin-stimulated FAK autophosphorylation and cytoskeletal rearrangement in cell spreading and migration." in: The Journal of cell biology, Vol. 160, Issue 1, pp. 137-46, (2003) (PubMed).

    Kim, Feldman: "Insulin-like growth factor I prevents mannitol-induced degradation of focal adhesion kinase and Akt." in: The Journal of biological chemistry, Vol. 277, Issue 30, pp. 27393-400, (2002) (PubMed).

    Kovacic-Milivojević, Roediger, Almeida, Damsky, Gardner, Ilić: "Focal adhesion kinase and p130Cas mediate both sarcomeric organization and activation of genes associated with cardiac myocyte hypertrophy." in: Molecular biology of the cell, Vol. 12, Issue 8, pp. 2290-307, (2001) (PubMed).

    Huan, van Adelsberg: "Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins." in: The Journal of clinical investigation, Vol. 104, Issue 10, pp. 1459-68, (1999) (PubMed).

    Clancy, Rediske, Tang, Nijher, Frenkel, Philips, Abramson: "Outside-in signaling in the chondrocyte. Nitric oxide disrupts fibronectin-induced assembly of a subplasmalemmal actin/rho A/focal adhesion kinase signaling complex." in: The Journal of clinical investigation, Vol. 100, Issue 7, pp. 1789-96, (1997) (PubMed).

  • Target
    FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
    Alternative Name
    FAK (PTK2 Products)
    Synonyms
    FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, PPP1R71 antibody, p125FAK antibody, pp125FAK antibody, PTK2 antibody, Fadk antibody, mKIAA4203 antibody, fak antibody, fak1a antibody, ptk2 antibody, ptk2a antibody, wu:fa18c04 antibody, zgc:66114 antibody, CG10023 antibody, CT28129 antibody, DFAK antibody, DFak antibody, DFak56 antibody, Dfak antibody, Dfak56 antibody, DmFAK antibody, Dmel\\CG10023 antibody, Fak56 antibody, Fak56D antibody, fak56 antibody, protein tyrosine kinase 2 antibody, protein tyrosine kinase 2 L homeolog antibody, PTK2 protein tyrosine kinase 2 antibody, protein tyrosine kinase 2ab antibody, Focal adhesion kinase antibody, PTK2 antibody, ptk2.L antibody, Ptk2 antibody, ptk2ab antibody, Fak antibody
    Background
    Focal Adhesion Kinase (FAK) is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the Focal Adhesion Targeting sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation and activation of FAK. This creates binding sites for SH2 domains of intracellular signaling molecules such as src, PI3 kinase, and Grb2. FAK's ability to bind numerous structural and signaling proteins via a variety of interactions has led to substantial speculation about its function. Although FAK's precise role has not been elucidated, proposed possibilities include regulating cell motility, cell growth, cytoskeletal organization, and adhesion-dependent cell survival.
    Synonyms: Focal Adhesion Kinase
    Molecular Weight
    116-125 kDa
    Pathways
    Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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