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PKC theta antibody (Protein Kinase C, theta) (AA 21-217)

Details for Product anti-PRKCQ Antibody No. ABIN967739, Supplier: Log in to see
Antigen
  • A130035A12Rik
  • AW494342
  • nPKC-theta
  • PKC-0
  • PKC-theta
  • Pkcq
  • PKCtheta
  • PRKCT
Alternatives
anti-Human PKC theta antibody for Western Blotting
Epitope
AA 21-217
41
41
37
10
9
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
194
117
108
4
4
4
4
Host
Mouse
203
6
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
This PKC theta antibody is un-conjugated
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
150
107
72
34
31
29
4
2
2
2
1
1
1
Supplier
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Immunogen Human PKCtheta aa. 21-217
Clone 27-PKCtheta
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PKC theta (PRKCQ Antibody Abstract)
Background The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members (delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+-independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCtheta transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes. PKCtheta mRNA is readily detectable in skeletal muscle, lung and brain. However, PKCtheta expression is not detected in several human carcinoma cell lines. Abundant expression of this PKC isozyme in hematopoietic cells suggest that it may have a role in growth and differentiation processes of these cells. This antibody is routinely tested by western blot analysis.
Molecular Weight 79 kDa
Research Area Cancer
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-PKC theta antibody (Protein Kinase C, theta) (AA 21-217) (ABIN967739) Western blot analysis of PKCtheta on a Jurkat lysate. Lane 1: 1:250, lane 2: 1:500, l...
Immunofluorescence (IF) image for anti-PKC theta antibody (Protein Kinase C, theta) (AA 21-217) (ABIN967739) Immunofluorescence staining of A431 cells.
 image for anti-PKC theta antibody (Protein Kinase C, theta) (AA 21-217) (ABIN967739) anti-Protein Kinase C, theta (PRKCQ) (AA 21-217) antibody (Image 3)
Western Blotting (WB) image for anti-PKC theta antibody (Protein Kinase C, theta) (AA 21-217) (ABIN967739) anti-Protein Kinase C, theta (PRKCQ) (AA 21-217) antibody (Image 4)
Product cited in: Villalba, Bi, Rodriguez, Tanaka, Schoenberger, Altman: "Vav1/Rac-dependent actin cytoskeleton reorganization is required for lipid raft clustering in T cells." in: The Journal of cell biology, Vol. 155, Issue 3, pp. 331-8, 2001

Dienz, Hehner, Droge, Schmitz: "Synergistic activation of NF-kappa B by functional cooperation between vav and PKCtheta in T lymphocytes." in: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 24547-51, 2000

Villalba, Coudronniere, Deckert, Teixeiro, Mas, Altman: "A novel functional interaction between Vav and PKCtheta is required for TCR-induced T cell activation." in: Immunity, Vol. 12, Issue 2, pp. 151-60, 2000

Soderling: "Protein kinases. Regulation by autoinhibitory domains." in: The Journal of biological chemistry, Vol. 265, Issue 4, pp. 1823-6, 1990

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988