Son of Sevenless Homolog 1 (Drosophila) (SOS1) (AA 1-109) antibody

Details for Product No. ABIN967740
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Antigen
Synonyms GF1, GGF1, GINGF, HGF, NS4, 4430401P03Rik, 9630010N06, AI449023
Epitope
AA 1-109
(2), (2), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(23), (7), (7), (4)
Host
Mouse
(17), (11), (3)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
(21), (17), (5), (4), (3), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 50 µg
Options
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Catalog No. ABIN967740
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Immunogen Mouse mSos1
Clone 25
Isotype IgG1
Cross-Reactivity Human, Dog (Canine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Sos1
Background The Sos (son of sevenless) gene was originally identified in Drosophila, and two mammalian homologues (mSos1 and mSos2) were isolated from a mouse cDNA library. These two cDNAs predict proteins that are approximately 70% identical in their amino acid residues. Both mSos1 and mSos2 are expressed in a wide number of mouse embryonic and adult tissues as well as in several cell lines. The human homologues, hSos1 and hSos2 have also been isolated and show a very high degree of amino acid identity to the mouse genes (98% for Sos1). Sos1 has a predicted molecular weight of 150kDa, but the apparent molecular weight is closer to 170 kDa, presumably due to a high proline content. The mammalian Sos1 protein has a highly specific guanine nucleotide exchange activity toward p21ras . In EGF-stimulated cells, Sos1 interacts with the SH3 domains of GRB2, and GRB2 binds via its SH2 domain to tyrosine 1068 of the activated EGF receptor. Thus, GRB2 recruits Sos1 to the plasma membrane and enables it to activate the Ras signaling pathway by enhancing GTP loading on p21ras .
Molecular Weight 170 kDa
Comment

Related Products: ABIN968539, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Son of Sevenless Homolog 1 (Drosophila) (SOS1) (AA 1-109) antibody Western blot analysis of Sos1 on PC12 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Sos1.
Product cited in: Egan, Giddings, Brooks et al.: "Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation." in: Nature, Vol. 363, Issue 6424, pp. 45-51, 1993 (PubMed).

Buday, Downward: "Epidermal growth factor regulates p21ras through the formation of a complex of receptor, Grb2 adapter protein, and Sos nucleotide exchange factor." in: Cell, Vol. 73, Issue 3, pp. 611-20, 1993 (PubMed).

Salojin, Zhang, Meagher et al.: "ZAP-70 is essential for the T cell antigen receptor-induced plasma membrane targeting of SOS and Vav in T cells." in: The Journal of biological chemistry, Vol. 275, Issue 8, pp. 5966-75, 2000 (PubMed).

Kardinal, Konkol, Lin et al.: "Chronic myelogenous leukemia blast cell proliferation is inhibited by peptides that disrupt Grb2-SoS complexes." in: Blood, Vol. 98, Issue 6, pp. 1773-81, 2001 (PubMed).

Furuta, Miura, Copeland et al.: "Light Chain 3 associates with a Sos1 guanine nucleotide exchange factor: its significance in the Sos1-mediated Rac1 signaling leading to membrane ruffling." in: Oncogene, Vol. 21, Issue 46, pp. 7060-6, 2002 (PubMed).

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