TRKB antibody (Neurotrophic Tyrosine Kinase, Receptor, Type 2) (AA 156-322)

Details for Product anti-NTRK2 Antibody No. ABIN967746, Supplier: Log in to see
Antigen
  • trkb
  • trkb-b
  • xtrkb
  • si:ch211-220b11.1
  • trkB2
  • NTRK2
  • ntrk2
  • gp145-trkb
  • TRKB
  • GP145-TrkB
  • trk-B
  • AI848316
  • C030027L06Rik
  • Tkrb
  • trkB
  • RATTRKB1
  • TRKB1
  • Trk-B
  • TrkB
  • neurotrophic tyrosine kinase, receptor, type 2
  • neurotrophic tyrosine kinase, receptor, type 2b
  • NTRK2
  • ntrk2-b
  • ntrk2b
  • ntrk2-a
  • ntrk2
  • Ntrk2
Alternatives
anti-Chicken TRKB antibody for Immunofluorescence
Epitope
AA 156-322
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Reactivity
Rat (Rattus)
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This TRKB antibody is un-conjugated
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Application
BioImaging (BI), Fluorescence Microscopy (FM), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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45
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Options
Supplier
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Supplier Product No.
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Immunogen Human TrkB aa. 156-322
Clone 47-TrkB
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name TrkB (NTRK2 Antibody Abstract)
Background The full-length TrkB gene has been reported to encode for a 145 kDa glycosylated transmembrane tyrosine kinase and neurotrophin receptor. The same gene also has been reported to encode for a 95 kDa glycoprotein that is identical to gp145 [TrkB] at the extracellular domain and transmembrane portion but lacks the intracellular portion. TrkB has been observable in a range of 116-145 kD and 70-95 kD due to various TrkB maturation states, subcellular localizations, and glycosylation states. TrkB belongs to a family of tyrosine kinases that include the TrkA proto-oncogene and TrkC. All have an extracellular ligand-binding domain, a transmembrane region, and intracellular kinase and autophosphorylation domains. TrkB binds the neurotrophins NT3 and NT4/5, as well as brain-derived neurotrophic factor (BDNF), a peptide that helps motor neuron survival and repair. The TrkB tyrosine kinase is activated upon binding to BDNF resulting in autophosphorylation of residues Y670, Y674 and Y675 and the subsequent association of several intracellular proteins like PLCgamma, Shc, and PI3-Kinase. TrkB is widely expressed in cells of neuroepithelium and neural crest origin. Some of these include motor neurons, dopamine-producing neurons, and neurons which release gamma-aminobutyric acid in the substantia nigra, neocortex, and hippocampus. The two TrkB gene products are differentially expressed in regions of the adult brain.
Synonyms: Neurotrophic Tyrosine Kinase Receptor Type 2, NTRK2
Molecular Weight 116-145 & 70-95 kDa
Research Area Cancer
Pathways RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 156-322) antibody (ABIN967746) Western blot analysis of TrkB on a rat cerebrum lysate (left). Lane 1: 1:250, lane 2:...
Immunofluorescence (IF) image for anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 156-322) antibody (ABIN967746) Immunofluorescent staining of undifferentiated (left) and differentiated (right) SH-S...
 image for anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 156-322) antibody (ABIN967746) anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 156-322) antibody (Image 3)
Western Blotting (WB) image for anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 156-322) antibody (ABIN967746) anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (AA 156-322) antibody (Image 4)
Product cited in: Du, Feng, Yang, Lu: "Activity- and Ca(2+)-dependent modulation of surface expression of brain-derived neurotrophic factor receptors in hippocampal neurons." in: The Journal of cell biology, Vol. 150, Issue 6, pp. 1423-34, 2000 (PubMed).

Muller, Djebbara-Hannas, Jourdain, Vutskits, Durbec, Rougon, Kiss: "Brain-derived neurotrophic factor restores long-term potentiation in polysialic acid-neural cell adhesion molecule-deficient hippocampus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 8, pp. 4315-20, 2000 (PubMed).

Jovanovic, Czernik, Fienberg, Greengard, Sihra: "Synapsins as mediators of BDNF-enhanced neurotransmitter release." in: Nature neuroscience, Vol. 3, Issue 4, pp. 323-9, 2000 (PubMed).

Kryl, Yacoubian, Haapasalo, Castren, Lo, Barker: "Subcellular localization of full-length and truncated Trk receptor isoforms in polarized neurons and epithelial cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 14, pp. 5823-33, 1999 (PubMed).

Armanini, McMahon, Sutherland, Shelton, Phillips: "Truncated and catalytic isoforms of trkB are co-expressed in neurons of rat and mouse CNS." in: The European journal of neuroscience, Vol. 7, Issue 6, pp. 1403-9, 1995 (PubMed).

Ebendal: "Function and evolution in the NGF family and its receptors." in: Journal of neuroscience research, Vol. 32, Issue 4, pp. 461-70, 1992 (PubMed).

Klein, Conway, Parada, Barbacid: "The trkB tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain." in: Cell, Vol. 61, Issue 4, pp. 647-56, 1990 (PubMed).

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