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Insulin Receptor antibody (INSR) (AA 1006-1144)

Details for Product anti-INSR Antibody No. ABIN967753, Supplier: Login to see
Antigen
  • 18402
  • CG18402
  • DIHR
  • DILR
  • DIR
  • DIRH
  • DIRbeta
  • DInR
  • DInr
  • Dir-a
  • Dir-b
  • Dmel\\CG18402
  • INR
  • INS
  • IR
  • Inr
  • Inr-alpha
  • Inr-beta
  • InsR
  • dINR
  • dIR
  • dIRH
  • dInR
  • dInr
  • dInsR
  • dinr
  • dir
  • er10
  • inr
  • insulin/insulin-like growth factor receptor
  • l(3)05545
  • l(3)93Dj
  • l(3)er10
  • lnR
  • INSR
  • NV14476
  • CD220
  • HHF5
  • 4932439J01Rik
  • D630014A15Rik
  • IR-A
  • IR-B
  • ir-A
  • ir
  • cd220
  • hhf5
  • CTK-1
Alternatives
anti-Mouse (Murine) Insulin Receptor antibody for Enzyme Immunoassay
Epitope
AA 1006-1144
32
26
21
20
15
10
9
7
7
6
6
5
5
5
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
Reactivity
Mouse (Murine), Rat (Rattus)
271
78
54
10
6
5
3
2
1
1
1
1
1
1
1
1
Host
Mouse
219
81
3
Clonality (Clone)
Monoclonal ()
Conjugate
This Insulin Receptor antibody is un-conjugated
21
16
9
9
7
7
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
166
122
52
44
32
18
18
11
10
10
10
8
8
7
2
2
1
1
1
Supplier
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Immunogen Human Insulin pro-receptor beta subunit 1006-1144
Clone 46-CD220
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD220 (INSR Antibody Abstract)
Background Insulin Receptor (IR) is a transmembrane receptor tyrosine kinase which, upon insulin binding, initiates a cascade of events, including autophosphorylation, phosphorylation of cellular protein substrates, glucose transport, and glycogen synthesis. IR is synthesized as a large glycosylated precursor that is cleaved upon maturation into a 130 kDa alpha-subunit with kinase activity and a 95 kDa beta-subunit. The active Insulin Receptor is a heterotetramer of homologus alpha and beta subunits joined by disulfide bonds. Among the major cytosolic substrates of the Insulin Receptor are IRS-1 and -2, beta-Adrenergic receptor, and pp15 (adipocyte lipid-binding protein, ALBP). Autophosphorylation of the IR recruits IRS-1 and -2 to the phosphotyrosines. Subsequently, the phosphorylated IRS-1 and -2 act as docking sites for other signaling proteins like PI3-Kinase, Shc, PTP1D, Nck, etc. In addition, the phosphatase LAR is tightly associated with the IR and LAR becomes activated after insulin stimulation dephosphorylating the IR and its substrates. Therefore, LAR provides a turn-off mechanism in insulin signaling. This antibody is routinely tested by western blot analysis.
Synonyms: Insulin Receptor beta
Molecular Weight 95 kDa
Pathways NF-kappaB Signaling, RTK Signaling, AMPK Signaling
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Insulin Receptor antibody (INSR) (AA 1006-1144) (ABIN967753) Western blot analysis of CD220 on a mouse liver lysate. Lane 1: 1:250, lane 2: 1:500,...
Immunofluorescence (IF) image for anti-Insulin Receptor antibody (INSR) (AA 1006-1144) (ABIN967753) Immunofluorescence staining of PFSK-1 cells (human neuroectodermal tumor line, ATCC C...
 image for anti-Insulin Receptor antibody (INSR) (AA 1006-1144) (ABIN967753) anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (Image 3)
Western Blotting (WB) image for anti-Insulin Receptor antibody (INSR) (AA 1006-1144) (ABIN967753) anti-Insulin Receptor (INSR) (AA 1006-1144) antibody (Image 4)
Product cited in: Shumay, Song, Wang et al.: "pp60Src mediates insulin-stimulated sequestration of the beta(2)-adrenergic receptor: insulin stimulates pp60Src phosphorylation and activation." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 3943-54, 2002 (PubMed).

Zundel, Swiersz, Giaccia: "Caveolin 1-mediated regulation of receptor tyrosine kinase-associated phosphatidylinositol 3-kinase activity by ceramide." in: Molecular and cellular biology, Vol. 20, Issue 5, pp. 1507-14, 2000 (PubMed).

Ebina, Ellis, Jarnagin et al.: "The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling." in: Cell, Vol. 40, Issue 4, pp. 747-58, 1985 (PubMed).

Yip, Jack: "Insulin receptors are bivalent as demonstrated by photoaffinity labeling." in: The Journal of biological chemistry, Vol. 267, Issue 19, pp. 13131-4, 1992 (PubMed).

Frattali, Treadway, Pessin: "Insulin/IGF-1 hybrid receptors: implications for the dominant-negative phenotype in syndromes of insulin resistance." in: Journal of cellular biochemistry, Vol. 48, Issue 1, pp. 43-50, 1992 (PubMed).