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Details for Product No. ABIN967753

Insulin Receptor (INSR) (AA 1006-1144) antibody

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Antigen
Synonyms
HHF5, CD220, 4932439J01Rik, D630014A15Rik, IR, IR-A, IR-B, ir-A, CTK-1, cd220, hhf5, 18402, CG18402, DIHR, DILR, DIR, DIRH, DIRbeta, DInR, DInr, Dir-a, Dir-b, Dmel\\CG18402, INR, INS, Inr, Inr-alpha,  ... show more
Epitope
»Alternatives AA 1006-1144
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Pubmed 5 references available
Catalog no. ABIN967753
Quantity 150 µg
Price
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Immunogen Human Insulin pro-receptor beta subunit 1006-1144
Clone BB10
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name CD220
Background Insulin Receptor (IR) is a transmembrane receptor tyrosine kinase which, upon insulin binding, initiates a cascade of events, including autophosphorylation, phosphorylation of cellular protein substrates, glucose transport, and glycogen synthesis. IR is synthesized as a large glycosylated precursor that is cleaved upon maturation into a 130 kDa alpha-subunit with kinase activity and a 95 kDa beta-subunit. The active Insulin Receptor is a heterotetramer of homologus alpha and beta subunits joined by disulfide bonds. Among the major cytosolic substrates of the Insulin Receptor are IRS-1 and -2, beta-Adrenergic receptor, and pp15 (adipocyte lipid-binding protein, ALBP). Autophosphorylation of the IR recruits IRS-1 and -2 to the phosphotyrosines. Subsequently, the phosphorylated IRS-1 and -2 act as docking sites for other signaling proteins like PI3-Kinase, Shc, PTP1D, Nck, etc. In addition, the phosphatase LAR is tightly associated with the IR and LAR becomes activated after insulin stimulation dephosphorylating the IR and its substrates. Therefore, LAR provides a turn-off mechanism in insulin signaling. This antibody is routinely tested by western blot analysis.
Synonyms: Insulin Receptor beta
Molecular Weight 95 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Product cited in: Frattali, Treadway, Pessin: "Insulin/IGF-1 hybrid receptors: implications for the dominant-negative phenotype in syndromes of insulin resistance." in: Journal of cellular biochemistry, Vol. 48, Issue 1, pp. 43-50, 1992 (PubMed).

Yip, Jack: "Insulin receptors are bivalent as demonstrated by photoaffinity labeling." in: The Journal of biological chemistry, Vol. 267, Issue 19, pp. 13131-4, 1992 (PubMed).

Ebina, Ellis, Jarnagin et al.: "The human insulin receptor cDNA: the structural basis for hormone-activated transmembrane signalling." in: Cell, Vol. 40, Issue 4, pp. 747-58, 1985 (PubMed).

Zundel, Swiersz, Giaccia: "Caveolin 1-mediated regulation of receptor tyrosine kinase-associated phosphatidylinositol 3-kinase activity by ceramide." in: Molecular and cellular biology, Vol. 20, Issue 5, pp. 1507-14, 2000 (PubMed).

Shumay, Song, Wang et al.: "pp60Src mediates insulin-stimulated sequestration of the beta(2)-adrenergic receptor: insulin stimulates pp60Src phosphorylation and activation." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 3943-54, 2002 (PubMed).

Alternatives for antigen "Insulin Receptor (INSR)", type "Antibodies"
Hosts (129), (91), (2)
Reactivities (209), (59), (55), (20), (19), (12), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Applications (127), (117), (61), (52), (26), (17), (12), (10), (10), (8), (6), (6), (3), (2), (1)
Conjugates (12), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (24), (17), (9), (9), (7), (6), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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