Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 1-194) antibody

Antigen: Signal Transducer and Activator of Transcription 1, 91kDa (STAT1)

Synonyms: ISGF-3, STAT91, DKFZp686B04100, AA408197, 2010005J02Rik, IMUP, H2RSP, IMUP-1, IMUP-2

Binding Site: AA 1-194

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN967761

Additional Information

Alternative name: Stat1

Immunogen: Human Stat1

Cross-Reactivity: Chicken, Dog (Canine), Frog, Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: Ig

Description: ISGF3 (interferon-stimulated gene factor 3) is the primary transcription activator induced by the binding of interferon to a specific cell surface receptor. Functional ISGF3 is a complex of four polypeptides of 113 kDa (Stat2), 91 kDa (Stat1alpha), 84 kDa (Stat1beta), and 48 kDa. The first three polypeptides contain both SH2 and SH3 domains in response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the cytoplasmic ISGF3 subunits become tyrosine-phosphorylated and are translocated to the nucleus. This results in the formation of an active complex with the 48 kDa protein. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). Thus, growth factors and cytokines appear to activate a common signal transduction pathway which leads to the phosphorylation and subsequent nuclear translocation of a group of latent cytoplasmic transcription factors.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 91/84 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from antiserum by negative adsorption and affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Stat1 on A431 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Stat1.

Immunofluorescent staining of human endothelial cells.

Publications

Product: Fu, Schindler, Improta et al.: "The proteins of ISGF-3, the interferon alpha-induced transcriptional activator, define a gene family involved in signal transduction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7840-3, 1992 (PubMed).

Fu, Zhang: "Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter." in: Cell, Vol. 74, Issue 6, pp. 1135-45, 1993 (PubMed).

Su, Kitagawa, Xue et al.: "Activation of Stat1 by mutant fibroblast growth-factor receptor in thanatophoric dysplasia type II dwarfism." in: Nature, Vol. 386, Issue 6622, pp. 288-92, 1997 (PubMed).

Chang, Peng, Fu: "Interleukin-4 mediates cell growth inhibition through activation of Stat1." in: The Journal of biological chemistry, Vol. 275, Issue 14, pp. 10212-7, 2000 (PubMed).

Lemmink, Tuyt, Knol et al.: "Identification of LIL-STAT in monocytic leukemia cells and monocytes after stimulation with interleukin-6 or interferon gamma." in: Blood, Vol. 98, Issue 13, pp. 3849-52, 2001 (PubMed).