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PTPN6 antibody (Protein-tyrosine Phosphatase 1C) (AA 492-597)

Details for Product anti-PTPN6 Antibody No. ABIN967766, Supplier: Log in to see
Antigen
  • DRPLA
  • HCP
  • hcp
  • HCPH
  • Hcph
  • HPTP1C
  • me
  • motheaten
  • PTP-1C
  • Ptp1C
  • Ptph6
  • SH-PTP1
  • SHP
  • SHP-1
  • Shp-1
  • SHP-1L
  • Shp1
  • SHP1
Alternatives
anti-Human PTPN6 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 492-597
33
22
13
9
9
6
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
182
80
75
7
2
2
1
1
1
Host
Mouse
116
35
31
Clonality (Clone)
Monoclonal ()
Conjugate
This PTPN6 antibody is un-conjugated
7
7
7
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
136
76
54
29
28
23
9
7
7
6
1
Supplier
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Immunogen Human PTP1C aa.. 492-597
Clone 52-PTP1C-SHP1
Isotype IgG1
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PTP1C (PTPN6 Antibody Abstract)
Background PTP1C (SH-PTP1, SHP-1, or HCP) is a protein-tyrosine phosphatase (PTP) that is expressed in epithelial and hematopoietic cells. It contains two N-terminal SH2 domains and a C-terminal phosphatase domain. While its distribution is primarily cytosolic, exposure to various stimuli induces movement of PTP1C to other cellular locations to allow interaction with its substrates. This enzyme associates with the IL-3, IL-4, erythropoietin, and stem cell factor receptors and may regulate their signaling pathways by dephosphorylation of their receptors or their downstream effectors. Motheaten mice, which possess a nonfunctional PTP1C gene, display multiple hematopoietic abnormalities including increased proliferation and activation of myeloid cells. Studies involving these mice have clearly implicated PTP1C in negative regulation of CSF-1 (colony stimulating factor-1) mitogenic signaling and have demonstrated a role for PTP1C as an intermediate between Ras and the MAP kinase pathway.
Molecular Weight 68 kDa
Pathways JAK-STAT Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-PTPN6 antibody (Protein-tyrosine Phosphatase 1C) (AA 492-597) (ABIN967766) Western blot analysis of PTP1C on Jurkat cell lysate. Lane 1: 1:500, lane 2: 1:1000, ...
Immunofluorescence (IF) image for anti-PTPN6 antibody (Protein-tyrosine Phosphatase 1C) (AA 492-597) (ABIN967766) Immunofluorescent staining on HL60 cells.
Product cited in: Duchene, Schanstra, Pecher, Pizard, Susini, Esteve, Bascands, Girolami: "A novel protein-protein interaction between a G protein-coupled receptor and the phosphatase SHP-2 is involved in bradykinin-induced inhibition of cell proliferation." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40375-83, 2002 (PubMed).

Keilhack, Müller, Böhmer, Frank, Weidner, Birchmeier, Ligensa, Berndt, Kosmehl, Günther, Müller, Birchmeier, Böhmer: "Negative regulation of Ros receptor tyrosine kinase signaling. An epithelial function of the SH2 domain protein tyrosine phosphatase SHP-1." in: The Journal of cell biology, Vol. 152, Issue 2, pp. 325-34, 2001 (PubMed).

Lund-Johansen, Davis, Bishop, de Waal Malefyt: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." in: Cytometry, Vol. 39, Issue 4, pp. 250-9, 2000 (PubMed).

Liu, Hill, Chernoff: "Direct binding of the proline-rich region of protein tyrosine phosphatase 1B to the Src homology 3 domain of p130(Cas)." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31290-5, 1997 (PubMed).

Kenner, Anyanwu, Olefsky, Kusari: "Protein-tyrosine phosphatase 1B is a negative regulator of insulin- and insulin-like growth factor-I-stimulated signaling." in: The Journal of biological chemistry, Vol. 271, Issue 33, pp. 19810-6, 1996 (PubMed).